Comprehensive and deep profiling of the plasma proteome with protein corona on zeolite NaY  

作　　者：Congcong Ma Yanwei Li Jie Li Lei Song Liangyu Chen Na Zhao Xueping Li Ning Chen Lixia Long Jin Zhao Xin Hou Li Ren Xubo Yuan 

机构地区：[1]Tianjin Key Laboratory of Composite and Functional Materials,School of Materials Science and Engineering,Tianjin University,Tianjin,300350,China [2]Department of Integrative Oncology,Tianjin Medical University Cancer Institute and Hospital and Key Laboratory of Cancer Prevention and Therapy,Tianjin,300060,China [3]Department of Proteomics,Tianjin Key Laboratory of Clinical Multi-omics,Tianjin,300308,China [4]Department of Clinical Laboratory,Tianjin Medical University Cancer Institute and Hospital,National Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin's Clinical Research Center for Cancer,Tianjin,300060,China

出　　处：《Journal of Pharmaceutical Analysis》2023年第5期503-513,共11页药物分析学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No:51773151)。

摘　　要：Proteomic characterization of plasma is critical for the development of novel pharmacodynamic biomarkers.However,the vast dynamic range renders the profiling of proteomes extremely challenging.Here,we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and deep profiling of the plasma proteome using the plasma protein corona formed on zeolite NaY.Specifically,zeolite NaY and plasma were co-incubated to form plasma protein corona on zeolite NaY(NaY-PPC),followed by conventional protein identification using liquid chromatography-tandem mass spectrometry.NaY was able to significantly enhance the detection of low-abundance plasma proteins,minimizing the“masking”effect caused by high-abundance proteins.The relative abundance of middleand low-abundance proteins increased substantially from 2.54%to 54.41%,and the top 20 highabundance proteins decreased from 83.63%to 25.77%.Notably,our method can quantify approximately 4000 plasma proteins with sensitivity up to pg/mL,compared to only about 600 proteins identified from untreated plasma samples.A pilot study based on plasma samples from 30 lung adenocarcinoma patients and 15 healthy subjects demonstrated that our method could successfully distinguish between healthy and disease states.In summary,this work provides an advantageous tool for the exploration of plasma proteomics and its translational applications.

关 键 词：NAY Plasma proteomics Protein corona Low-abundance proteins 

分 类 号：R285[医药卫生—中药学]