绢毛委陵菜PsWRKY40的克隆与耐镉功能分析  

Cloning and Cd-resistant Analysis of PsWRKY40 in Potentilla sericea

作　　者：高鹏飞 高冰 冯郑红 吴建慧[1] GAO Pengfei;GAO Bing;FENG Zhenghong;WU Jianhui(College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学园林学院,哈尔滨150040

出　　处：《园艺学报》2023年第6期1269-1283,共15页Acta Horticulturae Sinica

基　　金：黑龙江省自然科学基金项目(LH2020C045);中央高校基本科研业务费专项资金项目(2572019BK03)。

摘　　要：以绢毛委陵菜(Potentilla sericea)为材料,克隆得到PsWRKY40的c DNA全长序列。该基因的开放阅读框长978bp,编码325个氨基酸,属于WRKY家族GroupⅡ亚族。荧光定量PCR分析表明,PsWRKY40被NaCl、CdCl_(2)和甘露醇显著诱导表达。将PsWRKY40异源转化拟南芥,转基因植株在镉处理后,其对Cd的抗性显著高于野生型植株,AtSOD、AtPOD和AtCAT的表达量显著提高。上述结果表明PsWRKY40可被镉诱导,可能参与绢毛委陵菜抗镉调控。The full-length cDNA sequence of PsWRKY40 was cloned from Potentilla sericea.The open reading frame of this gene was 978 bp in length,which encoded 325 amino acids,and belonged to the GroupⅡsubfamily of the WRKY family.The expression level of PsWRKY40 significantly increased by NaCl,CdCl_(2) and mannitol treatments.PsWRKY40 was transformed into Arabidopsis thaliana heterologously,and transgenic plant was obtained.Under the treatment of Cd^(2+),it was found that the resistance of the transgenic lines was significantly higher than that of the wild type,and transgenic lines had a significantly higher expression level of AtSOD,AtPOD and AtCAT.The results showed that PsWRKY40 can be induced by Cd2+,so it may participate in the regulation of Cd2+resistance in Potentilla sericea.

关 键 词：绢毛委陵菜 WRKY 镉胁迫 基因克隆 功能分析 

分 类 号：S682[农业科学—观赏园艺]