不同养殖厂美洲大蠊提取物的化学成分分析及多成分含量测定  

Chemical Composition Analysis and Multicomponent Content Determination of Extracts of Periplaneta americana from Different Breeding Plants

作　　者：陆西星 朱玲 吴浩[3] 张成桂 杨永寿 陈志鹏[3] 刘衡 Lu Xixing;Zhu Ling;Wu Hao;Zhang Chenggui;Yang Yongshou;Chen Zhipeng;Liu Heng(Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali,Yunnan 671000;College of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Utilization,Yunnan University of Chinese Medicine,Kunming 650000,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]云南省昆虫生物医药研发重点实验室,云南大理671000 [2]云南中医药大学中药学院暨云南省南药可持续利用研究重点实验室,昆明650000 [3]南京中医药大学药学院,南京210023

出　　处：《大理大学学报》2023年第8期30-38,共9页Journal of Dali University

基　　金：国家自然科学基金项目(81860742,82060780);云南省科技计划项目(202001AT070021);云南省重大科技专项计划项目(202002AA100007);云南省科技人才和平台计划项目(202105AG070012)。

摘　　要：目的:探究美洲大蠊提取物PA的化学成分类型及含量。方法:采用显色鉴别法鉴定美洲大蠊提取物PA中的成分类型;采用紫外分光光度法测定美洲大蠊提取物PA中多肽、氨基酸、多糖、生物碱及酚类的含量,HPLC测定美洲大蠊提取物PA中尿嘧啶、肌苷、原儿茶酸的含量。结果:所建立各成分含量测定的方法具有良好的重复性和稳定性,20批次美洲大蠊提取物PA主要成分为多肽、氨基酸、多糖、生物碱和酚类,以及尿嘧啶、肌苷和原儿茶酸等成分,不同批次的美洲大蠊提取物PA主成分差异不明显。因采收时间和养殖条件的不同,不同厂家不同批次美洲大蠊提取物PA的成分含量存在一定差异。结论:通过分析美洲大蠊提取物PA的化学成分及含量,为后续药效物质的筛选及全面有效评价美洲大蠊药材的质量提供依据。Objective:To investigate the chemical constituents and contents of Periplaneta americana extract PA.Methods:The colorimetric method was used to identify the major components in P.americana extract PA.The contents of polypeptides,amino acids,polysaccharides,alkaloids,and phenols in P.americana extract PA were determined by ultraviolet spectrophotometry,while the contents of uracil,inosine,and protocatechuic acid in P.americana extract PA were determined by HPLC.Results:The established methods for the determination of each component had good reproducibility and stability.The main components of 20 batches of P.americana extract PA were polypeptides,amino acids,polysaccharides,alkaloids and phenols,as well as uracil,inosine and protocatechuic acid.There was no significant difference in the main components of different batches of P.americana extract PA.Because of the different harvest time and culture conditions,components of different batches of P.americana extract PA from different breeding plants had certain differences.Conclusion:By analyzing the material composition and content of P.americana extract PA,this study provides a basis for the subsequent screening of functional substances and comprehensive and effective evaluation of the quality of P.americana.

关 键 词：美洲大蠊提取物 显色鉴别 紫外分光光度法 HPLC 

分 类 号：R284.1[医药卫生—中药学]