香花油茶不同无性系叶表型性状及叶绿素含量的差异  

Analysis of leaf phenotypic characters and leaf phenotypic character of different clones in Camellia osmantha

作　　者：陈梦秋 张日清[1] 蔡娅 程俊媛 尹显明 叶航[2,3] CHEN Mengqiu;ZHANG Riqing;CAI Ya;CHENG Junyuan;YIN Xianming;YE Hang(Central South University of Forestry&Technology,Changsha 410004,Hunan,China;Guangxi Key Laboratory of Special Non-wood Forest Cultivation&Utilization,Guangxi Forestry Research Institute,Nanning 530002,Guangxi,China;Improved variety and cultivation engineering Research Center of Oil-tea Camellia in Guangxi,Guangxi Forestry Research Institute,Nanning 530002,Guangxi,China)

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]广西壮族自治区林业科学研究院,广西特色经济林培育与利用重点实验室,广西南宁530002 [3]广西壮族自治区林业科学研究院,广西油茶良种与栽培工程技术研究中心,广西南宁530002

出　　处：《经济林研究》2023年第2期102-109,共8页Non-wood Forest Research

基　　金：广西创新驱动发展专项“典型栽培区油茶绿色栽培模式集成应用示范”(桂科AA20302021-4)和“油茶园艺化栽培关键技术研究”(桂科AA20302021-2)。

摘　　要：【目的】为香花油茶良种选育和生态经济型品种筛选提供参考。【方法】以生长良好的30个香花油茶无性系叶为材料,使用叶面积仪和Dualex植物多酚-叶绿素仪对叶表型性状和叶绿素含量进行测定。【结果】叶水分含量特征:叶含水量为52.07%~62.44%,含水量高于55%的无性系占比70%以上。叶表型性状:15号无性系的叶长最大,29号无性系的叶宽、叶面积、叶周长、形状系数均最小;各无性系叶表型性状的变异系数为9.18%~23.07%,叶周长的变异系数最小(为9.18%),叶面积的变异系数最大(为23.07%);叶宽、叶面积与其他叶表型性状间均存在极显著相关性;在主成分分析中,前2个主成分的累计贡献率达到92.708%,说明这2个主成分概括了6个性状的主要信息。其中,叶面积的特征向量值最大,其次是叶宽,叶面积和叶宽是区分香花油茶无性系最主要的叶性状。经聚类分析,香花油茶各无性系可被划分为3个类群。叶绿素含量:香花油茶不同无性系间叶绿素含量存在变异,变异系数为8.48%;25号无性系叶基、叶中的叶绿素含量与其他无性系有显著差异,叶尖叶绿素含量除与18号无性系无显著差异外,与其他无性系均有显著差异。【结论】香花油茶叶表型性状的遗传差异较大;叶中的叶绿素含量与叶总体的叶绿素含量的相关性最高,即叶中部可作为测定叶片叶绿素含量的最佳部位。【Objective】To provide reference for the breeding of fine varieties and screening of eco-economic varieties of Camellia osmantha.【Method】The leaf phenotypic traits and chlorophyll content of 30 C.osmantha clones with good growth were measured by leaf area meter and Dualex plant polyphenol chlorophyll meter.【Result】Leaf water content characteristics:leaf water content was from 52.07%to 62.44%,and the clones with water content higher than 55%accounted for more than 70%.Leaf phenotypic traits:the leaf length of clone 15 was the largest,and the leaf width,leaf area,leaf circumference and shape coefficient of clone 29 were the smallest.The coefficient of variation of leaf phenotypic traits of each clone was from 9.18%to 23.07%,the coefficient of variation of leaf perimeter was the smallest,which was 9.18%,and the coefficient of variation of leaf area was the largest,which was 23.07%.There were significant correlations between leaf width,leaf area and other leaf phenotypic traits.In the principal component analysis,the cumulative contribution rate of the first two principal components reached 92.708%,indicating that two principal components summarized the main information of the six traits.Among them,the load of leaf area was the largest,followed by leaf width,which was the most important leaf trait to distinguish C.osmantha clones.By cluster analysis,the clones of C.osmantha could be divided into three groups.Chlorophyll content:there was a variation in chlorophyll content among different clones of C.osmantha,and the coefficient of variation was 8.48%.The chlorophyll content in leaf base and leaf of clone 25 was significantly different from that of other clones,and the chlorophyll content in leaf tip was significantly different from that of other clones except for no significant difference with clone 18.【Conclusion】The genetic difference of phenotypic traits of C.osmantha is larger;The chlorophyll content in leaves have the highest correlation with the overall chlorophyll content in leaves,which means t

关 键 词：香花油茶 无性系 叶的水分含量特征 叶表型性状 叶绿素含量 叶位 

分 类 号：S601[农业科学—园艺学]