呼吸道合胞病毒诱导人肺腺癌细胞中TLR3的表达及其在儿童肺炎诊断中的意义  

Expression of TLR3 in human adenocarcinoma of the lung cells induced by respiratory syncytial virus and its significance in the diagnosis of pneumonia in children

作　　者：刘冬梅[1] 王亚楠[1] 马彤辉 王敬敏[1] 王华[1] 马丹丹 侯秀玲[1] 董巧丽[2] Liu Dongmei;Wang Yanan;Ma Tonghui;Wang Jingmin;Wang Hua;Ma Dandan;Hou Xiuling;Dong Qiaoli(Department of Pediatric,Baoding Second Central Hospital,Baoding 072750,China;Department of Pediatric,Affiliated Hospital of Hebei University,Baoding 071000,China)

机构地区：[1]保定市第二中心医院儿科,保定072750 [2]河北大学附属医院儿科,保定071000

出　　处：《中国医师杂志》2023年第6期859-863,共5页Journal of Chinese Physician

基　　金：河北省医学科学研究重点课题计划(20181791)。

摘　　要：目的研究呼吸道合胞病毒(RSV)诱导人肺腺癌细胞中Toll样受体3(TLR3)的表达及其在儿童肺炎诊断中的意义。方法将A549细胞分成RSV感染组[加入1μg/ml脂多糖(TLR3激动剂)150μl干预后转染RSV病毒]、脂多糖刺激组(加入1μg/ml脂多糖150μl干预)和正常对照组(正常培养),比较不同组别A549细胞肿瘤坏死因子-α、白细胞介素-8、TLR3蛋白和TLR3 mRNA表达。前瞻性选择保定市第二中心医院2019年8月至2021年10月收治的80例RSV感染性肺炎患儿为RSV肺炎组,60例普通肺炎患儿为普通肺炎组,本院体检的60例健康儿童为对照组,比较不同组别血清TLR3 mRNA表达,应用ROC曲线评估血清TLR3对RSV肺炎的诊断效能。结果不同组别A549细胞中TLR3蛋白表达差异有统计学意义(P<0.001)。不同组别A549细胞在不同时点TLR3 mRNA的表达差异均有统计学意义(均P<0.001)。不同组别A549细胞在不同时点肿瘤坏死因子-α和白细胞介素-8的表达差异均有统计学意义(均P<0.05)。三组受试者血清TLR3 mRNA的表达差异有统计学意义(F=155.237,P<0.001)。TLR3 mRNA诊断RSV肺炎的临界值为66.87,其对应的灵敏度为73.75%,特异度为70.83%,曲线下面积(AUC)为0.803(95%CI:0.753~0.855)。结论呼吸道合胞病毒诱导人肺癌细胞,可通过TLR3表达促进疾病发病;血清TLR3可应用于RSV肺炎诊断。Objective To study the expression of Toll like receptor 3(TLR3)in human adenocarcinoma of the lung cells induced by respiratory syncytial virus(RSV)and its significance in the diagnosis of pneumonia in children.Methods A549 cells were divided into RSV infection group[added 1μg/ml Lipopolysaccharide(TLR3 agonist)transfected RSV virus after 150μl intervention],Lipopolysaccharide stimulation group(added 1μg/ml Lipopolysaccharide 150μl intervention)and normal control group(normal culture).The mRNA expressions of tumor necrosis factor-α,interleukin 8,TLR3 protein and TLR3 in A549 Cells of different groups were compared.We prospectively selected 80 children with RSV infectious pneumonia admitted to Baoding Second Central Hospital from August 2019 to October 2021 as the RSV pneumonia group,and sixty children with common pneumonia were taken as the common pneumonia group,and 60 healthy children in our hospital were taken as the control group.The mRNA expression of serum TLR3 in different groups was compared,and the diagnostic efficacy of serum TLR3 in RSV pneumonia was evaluated by receiver operating characteristic.Results There was a statistically significant difference in the expression of TLR3 protein among different groups of A549 cells(P<0.001).The expression differences of TLR3 mRNA in different groups of A549 cells at different time points were statistically significant(all P<0.001).There was significant difference in the expression of tumor necrosis factor-αand interleukin 8 of A549 cells at different time points in different groups(all P<0.05).There was a statistically significant difference in the expression of serum TLR3 mRNA among the three groups of subjects(F=155.237,P<0.001).The critical value for TLR3 gene diagnosis was 66.87,with corresponding sensitivity of 73.75%,specificity of 70.83%,and the area under curve(AUC)of 0.803(95%CI:0.753-0.855).Conclusions Respiratory syncytial virus induces human lung cancer cells and promotes disease progression through TLR3 expression;Serum TLR3 can be used for th

关 键 词：呼吸道合胞病毒 A549细胞 TOLL样受体3 肺炎 

分 类 号：R725.6[医药卫生—儿科]