姜酚纯化前后对胰脂肪酶抑制活性、抗氧化活性比较研究  

Comparative Study on Pancreatic Lipase Inhibitory Activity and Antioxidant Activity of Gingerol Before and After Purification

作　　者：徐菁 王秀芬 刘晓燕 XU Jing;WANG Xiufen;LIU Xiaoyan(School of Food and Pharmaceutical Engineering,Guiyang Normal University,Guiyang 550005,China;Guizhou Fruit Processing Technology Engineering Research Center,Guiyang Normal University,Guiyang 550005,China)

机构地区：[1]贵阳学院食品与制药工程学院,贵阳550005 [2]贵阳学院贵州省果品加工工程技术研究中心,贵阳550005

出　　处：《中国农业科技导报》2023年第5期96-105,共10页Journal of Agricultural Science and Technology

基　　金：贵州省科技厅科技支撑项目(黔科合支撑[2020]1Y157);贵阳市科技局贵阳学院专项资金项目(GYU-KY-[2021])。

摘　　要：为了探讨姜酚的大孔树脂纯化方法,选择4种不同极性树脂(NKA-9、AB-8、D4020和D101)作静态吸附-解吸动力学研究。结果表明,AB-8为最佳树脂,以AB-8树脂为吸附剂,对其最佳纯化工艺进行探讨,并比较纯化前后姜酚对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-trinitrophenylhydrazine,DPPH)自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonicacid)diammonium,ABTS)自由基等的清除能力和对胰脂肪酶抑制活性,采用高效液相色谱法测定纯化前粗提物和纯化后纯化物中6-姜酚、8-姜酚、10-姜酚含量。结果表明,AB-8树脂动态纯化姜酚最佳条件为:上样质量浓度1.6mg·mL^(-1),pH=4,上样流速1.5 mL·min^(-1),以80%乙醇溶液流速1.5 mL·min^(-1)洗脱,经纯化后姜酚纯度为纯化前的3.3倍,高效液相色谱分析结果表明3种姜酚类单体物质含量相比纯化前提高了3倍以上,纯化后的姜酚对猪胰脂肪酶的抑制率和自由基清除能力明显高于纯化前,说明AB-8树脂对姜酚有较好的分离纯化作用,能够实现成分的富集,对提高姜酚的利用附加值具有重要意义。In order to explore the purification method of gingerol by macroporous resin,four different polar resins(NKA-9,AB-8,D4020 and D101)were selected to study the static adsorption-desorption kinetics.The results showed that AB-8 was the best resin which was used as adsorbent.The optimum purification process of gingerol was discussed,and the scavenging ability of gingerol to 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radical,2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium(ABTS)radical and its inhibitory activity to pancreatic lipase were compared before and after purification.The contents of 6-gingerol,8-gingerol and 10-gingerol in the crude extract before purification and after purification were determined by HPLC.The results showed that the optimum conditions for dynamic purification of gingerol by AB-8 resin were as follows:sample mass concentration 1.6 mg·mL^(-1),pH=4,sample flow rate 1.5 mL·min^(-1),elution rate 1.5 mL·min^(-1) at 80%ethanol solution.The purity of gingerol after purification was increased by 3.3 times.The results of high performance liquid chromatography showed that the content of three gingerol monomers was more than 3 times higher than that before purification.The inhibition rate and free radical scavenging ability of purified gingerol on porcine pancreatic lipase were significantly higher than those before purification,indicating that AB-8 resin had a good effect on the separation and purification of gingerol and could achieve the enrichment of components,which was of great significance to improve the added value of gingerol utilization.

关 键 词：大孔树脂 姜酚 吸附 解吸 抗氧化 抑制胰脂肪酶 

分 类 号：Q946[生物学—植物学]