莠去津降解菌的分离及不同施用方式对土壤修复效果的影响  

Isolation of Atrazine Degrading Bacteria and the Effect of Application Methods on Soil Remediation

作　　者：王琳琳[1] 李雪飞 李凤 周方园 张广志 张新建 WANG Linlin;LI Xuefei;LI Feng;ZHOU Fangyuan;ZHANG Guangzhi;ZHANG Xinjian(Shandong Provincial Key Laboratory of Applied Microbiology/Ecology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103;Linyi Natural Resources Development Service Center,Linyi,Shandong 276037)

机构地区：[1]齐鲁工业大学(山东省科学院)生态研究所/山东省应用微生物重点实验室,济南250103 [2]临沂市自然资源开发服务中心,山东临沂276037

出　　处：《中国农学通报》2023年第18期86-93,共8页Chinese Agricultural Science Bulletin

基　　金：山东省科技发展计划项目“防病促生型阿特拉津除草剂污染土壤修复菌剂的研发”(2013GNC11019)。

摘　　要：土壤中的莠去津残留会对大豆等后茬敏感作物造成药害,还会对人体和环境造成危害。为建立莠去津污染土壤高效修复技术,本研究进行了莠去津降解菌株的分离筛选,并比较了不同施用方式对土壤修复效果的影响。以莠去津为降解底物,从长期施用该农药的农田土壤中分离降解菌,根据菌落形态特征、16S rRNA基因序列和系统发育分析,对降解菌进行鉴定。通过发芽试验,验证降解菌对莠去津抑制大豆种子萌发的缓解作用。通过盆栽试验,比较了播前冲施、浸种和播后冲施对降解菌缓解莠去津药害效果的影响。结果表明:本研究筛选获得了一株莠去津高效降解菌Zatd001,鉴定结果为类节杆菌(Paenarthrobacter sp.)。菌株Zatd001能有效缓解莠去津对大豆种子萌发的抑制作用,3种施用方式都能显著降低莠去津对大豆植株的毒害,各生长指标与空白对照无显著差异。3种施用方式修复效果大小顺序为:播前冲施>播后冲施>浸种处理,但各施用方式差异不显著。上述结果表明,菌株Zatd001能有效缓解莠去津残留会对大豆造成药害,且施用方式灵活方便,该研究对降解菌在修复莠去津污染土壤中的应用具有重要实践指导意义。The residue of atrazine in soil will cause injury to soybean and other sensitive crops,as well as harm to the human body and environment.To establish efficient remediation techniques for atrazine-contaminated soil,this study carried out the isolation and screening of atrazine-degrading bacteria strains,and compared the effects of different application methods on soil remediation.Using atrazine as substrate,the degrading bacteria were isolated from soil samples with long-term application of atrazine.The degrading bacteria were identified based on colony morphology,16S rRNA gene sequence and phylogenetic analysis.Through the germination test,the alleviating effect of degrading bacteria on the inhibition of soybean germination by atrazine was verified.Pot experiment was conducted to compare the effects of different application methods on alleviating atrazine harm,including flushing application before sowing(Pre),seed soaking(SS)and flushing application after sowing(Post).The results showed that a highly efficient atrazine degrading strain Zatd001 was screened and identified as Paenarthrobacter sp.The strain Zatd001 could effectively alleviate the inhibition of atrazine on soybean seed germination.All the three application methods could significantly reduce the toxicity of atrazine to soybean plants,and there was no significant difference in soybean growth indexes between treated and blank groups.The order of repair effect of the three application methods was:Pre>Post>SS,but the differences among the three methods were not significant.These results indicate that strain Zatd001 could effectively alleviate the harm caused by atrazine residue to soybean,and its application method is flexible and convenient.This study has important practical significance for the application of degrading bacteria in the remediation of atrazine contaminated soil.

关 键 词：莠去津 降解菌 施用方式 土壤修复 大豆 

分 类 号：X592[环境科学与工程—环境工程]