2种黑籽南瓜响应枯萎病菌侵染的转录组学研究  

Two Varieties of Cucurbita ficifolia:Transcriptomic Studies in Response to Fusarium oxysporum Infection

作　　者：张浩 李海玉 施松梅 张忠润 唐榕梓 弓月芳 邓文鑫 杨正安[1] ZHANG Hao;LI Haiyu;SHI Songmei;ZHANG Zhongrun;TANG Rongzi;GONG Yuefang;DENG Wenxin;YANG Zhengan(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650500)

机构地区：[1]云南农业大学园林园艺学院,昆明650500

出　　处：《中国农学通报》2023年第18期107-116,共10页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金“枯萎病菌胁迫下的黑籽南瓜抗性基因表达差异及NBS类关键基因功能鉴定”(K1400245000)。

摘　　要：为了筛选白皮黑籽南瓜和绿皮花纹黑籽南瓜抗枯萎病的差异表达基因,挖掘参与抗性相关的代谢通路。以尖孢镰刀菌侵染2 d后和未侵染的2种幼苗叶片作为材料,利用Illumina Hiseq 2500测序技术进行转录组测序分析。以未侵染的幼苗作为对照,白皮和绿皮花纹黑籽南瓜差异表达基因分别有2082和1116个。其中141个基因在2种黑籽南瓜中均呈现出差异表达,62个基因具有相同表达趋势。对差异表达基因进行GO功能注释,结果显示2种黑籽南瓜差异表达基因主要富集在生物学过程中。KEGG代谢通路分析发现白皮有22个差异表达基因显著富集在植物激素信号通路,绿皮花纹有30个差异表达基因富集在苯丙烷生物合成通路。本研究挖掘出黑籽南瓜抗枯萎病差异表达基因,预测了抗性通路,为瓜类抗病机制的深入研究奠定了理论基础。To screen the differentially expressed genes for resistance to Fusarium wilt in two cultivated varieties of black-seeded pumpkin(Cucurbita ficifolia)named‘White Peel’and‘Green Peel with White’,and explore metabolic pathway involved in resistance,two varieties seedling leaves infected by Fusarium oxysporum for 2 days and uninfected by Fusarium oxysporum were used as materials,and transcriptome sequencing was performed by using Illumina Hiseq 2500 sequencing technology.Compared to the uninfected seedlings,there were 2082 and 1116 differentially expressed genes in‘White Peel’and‘Green Peel with White’,respectively.Among them,141 genes showed differential expression,and 62 genes had the same expression trend.Go functional annotation to differentially expressed genes showed that they were mainly enriched in biological processes.KEGG metabolic pathway analysis revealed that 22 differentially expressed genes were significantly enriched in the plant hormone signaling pathway in the‘White Peel’,while 30 differentially expressed genes were enriched in the phenylpropane biosynthesis pathway in‘Green Peel with White’.In this study,differentially expressed genes for resistance to Fusarium wilt in Cucurbita ficifolia were identified and the resistance pathway was predicted,which laid a theoretical foundation for further research on the resistance mechanism of melons.

关 键 词：黑籽南瓜 尖孢镰刀菌 抗病机制 转录组测序 差异表达基因 

分 类 号：S-3[农业科学]