贝莱斯芽孢杆菌(Bacillus velezensis)YFB3-1高密度液体发酵条件优化  

Bacillus velezensis YFB3-1:Optimization of Liquid Fermentation Conditions for High Density

作　　者：鲁耀雄[1,2] 李魏[2] 周瑚 瞿玉国 熊有明 王运生[2] LU Yaoxiong;LI Wei;ZHOU Hu;QU Yuguo;XIONG Youming;WANG Yunsheng(Institute of Agri-Environment and Ecology,Hunan Academy of Agricultural Sciences,Changsha 410125;College of Plant Protection,Hunan Agricultural University,Changsha 410128;Yueyang Inspection and Testing Center,Yueyang,Hunan 414000;Guzhang County Characteristic Industry Service Center,Guzhang,Hunan 416300;Hengyang Company of Hunan Tobacco Corporation,Hengyang,Hunan 421099)

机构地区：[1]湖南省农业科学院农业环境生态研究所,长沙410125 [2]湖南农业大学植物保护学院,长沙410128 [3]岳阳市检验检测中心,湖南岳阳414000 [4]古丈县特色产业服务中心,湖南古丈416300 [5]湖南省烟草公司衡阳市公司,湖南衡阳421099

出　　处：《中国农学通报》2023年第18期123-130,共8页Chinese Agricultural Science Bulletin

基　　金：湖南省自然科学基金项目“蚯蚓媒介Bacillus sp.YFB3-1缓解百合连作障碍病害发生的微生物机制研究”(2020JJ4393);农业农村部废弃物肥料化利用重点实验开放基金“稻耳轮作制度下的农业废弃物高效循环利用技术研究与示范”(KLFAW202004);长沙市自然科学基金项目“蚯蚓联合Bacillus velezensis YFB3-1对连作辣椒疫病的防治效果及机制研究”(kq2208105)。

摘　　要：旨在获得高密度的贝莱斯芽孢杆菌(Bacillus velezensis)YFB3-1生防菌剂,用于预防作物土传病害的发生。采用单因素和正交试验对菌株YFB3-1的液体培养基和培养条件进行优化。在酵母粉8 g/L、复合氨基酸10 g/L、氯化钠1 g/L、硫酸镁(MgSO_(4)·7H_(2)O)1 g/L的液体培养基中培养菌株YFB3-1,初始pH7.0、接种量2.5%、发酵温度30℃、转速180 r/min和培养时间48 h为发酵条件时,获得的菌体浓度为2.89×10^(10)cfu/mL,是菌株YFB3-1液体发酵的最优培养基和培养条件,比牛肉膏蛋白胨液体培养基(NB)的菌体浓度4.77×10^(9)cfu/mL,提高了5.06倍,在相同时间内可以提高发酵效率。本研究为生防菌B velezensis YFB3-1的产业化扩大培养和推广应用提供了理论依据和技术参数。The aim is to obtain high density Bacillus velezensis YFB3-1 biocontrol agent which can be used to prevent soil-borne diseases of crops.In this study,single factor and orthogonal experiments were used to optimize the liquid medium and culture conditions of strain YFB3-1.The results showed that strain YFB3-1 was cultured in liquid medium with yeast powder 8 g/L,compound amino acid 10 g/L,sodium chloride 1 g/L and magnesium sulfate(MgSO_(4)·7H_(2)O)1 g/L,and when the fermentation conditions were initial pH of 7.0,inoculation amount of 2.5%,fermentation temperature of 30℃,rotation speed of 180 r/min and culture time of 48 h,the bacterial concentration was 2.89×10^(10) cfu/mL,and the optimal culture medium and culture conditions were obtained for the liquid fermentation of strain YFB3-1.Compared with beef extract peptone liquid medium(NB),in which the bacterial concentration was 4.77×10^(9) cfu/mL,the bacterial concentration under the fermentation conditions of this experiment increased by 5.06 times,and the fermentation efficiency could be improved in the same time.The study provides a theoretical basis and technical parameters for the industrial expansion and application of B.velezensis YFB3-1.

关 键 词：贝莱斯芽孢杆菌 正交试验 高密度液体发酵 发酵条件优化 

分 类 号：Q93[生物学—微生物学]