比较转录组分析花生种子休眠调控网络  

Comparative transcriptome profiling of dormancy regulatory network in peanut

作　　者：王菲菲 张胜忠 胡晓辉[1] CHU Ye 崔凤高[1] 钟文[3] 赵立波 张天雨 郭进涛 于豪谅 苗华荣[1] 陈静[1] WANG Fei-Fei;ZHANG Sheng-Zhong;HU Xiao-Hui;CHU Ye;CUI Feng-Gao;ZHONG Wen;ZHAO Li-Bo;ZHANG Tian-Yu;GUO Jin-Tao;YU Hao-Liang;MIAO Hua-Rong;CHEN Jing(Shandong Peanut Research Institute,Qingdao 266100,Shandong,China;Department of Horticulture,University of Georgia Tifton Campus,Tifton 31793,GA,United States;Shandong Seed Administration Station,Jinan 250100,Shandong,China;Qingdao Agricultural Technology Extension Center,Qingdao 266071,Shandong,China;Agricultural Service Center of Hezhuang,Xinzheng,Zhengzhou 451150,Henan,China;Yantai Fenglin Foodstuff Co.,Ltd,Yantai 264108,Shandong,China)

机构地区：[1]山东省花生研究所,中国山东青岛266100 [2]佐治亚大学蒂芙顿校区园艺系,美国佐治亚州蒂芙顿31793 [3]山东省种子管理总站,中国山东济南250100 [4]青岛市农业技术推广中心,中国山东青岛266071 [5]新郑市和庄农业服务中心,中国河南新郑451150 [6]烟台枫林食品股份有限公司,中国山东烟台264108

出　　处：《作物学报》2023年第9期2446-2461,共16页Acta Agronomica Sinica

基　　金：国家自然科学基金青年基金项目(32001584,32201876);山东省自然科学基金面上项目(ZR2022MC045);山东省农业良种工程(2020LZGC001);山东省农业科学院创新工程(CXGC2022A03,CXGC2022A21);青岛市民生科技计划项目(20-3-4-26-nsh);新疆维吾尔自治区重大科技专项(2022A02008-3)资助。

摘　　要：种子休眠性是花生重要且复杂的农艺性状,对花生(ArachishypogaeaL.)的产量和品质影响巨大。为深入揭示花生种子休眠维持和解除的分子调控网络,本研究以强休眠品种花育52号(HY52)和弱休眠突变株系M23、M67为试材,种子吸胀处理(0 h、12 h、24 h)后测定其激素ABA和GA含量并进行转录组测序。吸胀12 h时M23和M67中GA含量显著高于HY52, ABA含量和ABA/GA比值则低于HY52。测序共得到31,373个差异表达基因(DEGs),其中ABA和GA生物合成和信号转导相关的基因在种子休眠维持和解除过程中发生显著变化,挖掘到50个ABA相关基因、8个GA相关基因、49个乙烯相关基因和13个生长素相关基因。此外,还鉴定到许多参与碳水化合物和脂质代谢、氨基酸代谢途径相关的DEG,挖掘到糖代谢相关基因5个、脂质代谢相关基因4个;昼夜节律调控也可能参与花生种子休眠解除。这表明,花生种子休眠维持和解除的调控是一个复杂网络,植物激素平衡调控可能只是其中一个重要部分。Seed dormancy is an important and complex agronomic trait affecting yield and quality of peanut(Arachis hypogaea L.).Seed dormancy and germination was reported to be regulated by the balance between abscisic acid(ABA)and gibberellic acid(GA).In this study,transcriptomic sequencing was performed with Huayu 52(HY52),a peanut cultivar with strong dormancy,and two EMS mutant lines from HY52 with a weak level of dormancy.Seeds from these three lines were imbibed for 0,12,and 24 h before tissue harvesting and RNA seq analysis.GA content of M23 and M67 was significantly higher than HY52 at 12 h after imbibition,however,the ABA content and ABA/GA ratio were lower than HY52.A total of 31,374 differentially expressed genes(DEGs)including biosynthesis and signal transduction related genes of plant hormones such as ABA and GA were discovered.We identified 50 genes related to ABA,8 genes related to GA,49 genes related to ethylene,and 13 genes related to auxin.Expression profiles of ABA and GA related genes was consistent with the higher GA and lower ABA content in the mutants compared with HY52 after 12 h and 24 h imbibition.In addition,many DEGs involved in carbohydrate and lipid metabolism,amino acid metabolism,and glutathione metabolism pathway were also identified.There were 5 carbohydrate metabolism related genes(GPT)and 4 lipid metabolism related genes.In addition,differentially regulated circadian rhythm pathways were found to involve in the process of peanut seed dormancy release.These results suggested that the regulation of dormancy maintenance and release was more complicated than phytohormone balance.

关 键 词：花生 休眠维持 休眠解除 转录组 植物激素 氨基酸代谢 

分 类 号：S565.2[农业科学—作物学]