378份甘薯引进种遗传多样性及群体结构分析  

Genetic diversity and population structure analysis of 378 introduced sweetpotato germplasm collections

作　　者：苏一钧 赵路宽 唐芬 戴习彬 孙亚伟[1] 周志林[1] 刘亚菊[1] 曹清河[1,2,3] SU Yi-Jun;ZHAO Lu-Kuan;TANG Fen;DAI Xi-Bin;SUN Ya-Wei;ZHOU Zhi-Lin;LIU Ya-Ju;CAO Qing-He(Jiangsu Xuhuai Regional Agricultural Academy of Sciences/Sweetpotato Research Institute,China Agricultural Academy of Sciences,Xuzhou 221131,Jiangsu,China;School of Life Sciences,Jiangsu Normal University,Xuzhou 221116,Jiangsu,China;Department of Horticulture,Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所/中国农业科学院甘薯研究所,江苏徐州221131 [2]江苏师范大学生命科学学院,江苏徐州221116 [3]海南大学园艺学院,海南海口570228

出　　处：《作物学报》2023年第9期2582-2593,共12页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-10-GW01-2022);国家自然科学基金国际(地区)合作与交流项目(31461143017);农业农村部物种资源保护项目(19210138);江苏省根茎类种质资源库项目(JSGB2018-03);江苏省种业振兴揭榜挂帅项目(JBGS[2021]010)资助。

摘　　要：甘薯起源于中南美洲,国外引进种不仅能够提升我国资源总量而且将会极大改变甘薯遗传基础狭窄的育种瓶颈。本文利用30对Simple sequence repeat (SSR)引物通过高通量核酸片段分析仪对378份甘薯种质资源进行基因分型,鉴定了国家甘薯种质资源试管苗库(徐州)中的引进种种质,分析了引进种之间的亲缘关系,为甘薯引进种的利用和种质创新提供理论依据。利用PROSize3.0软件读取条带,获得01数据库,将01数据导入Darwin软件计算Jaccard遗传距离矩阵,利用MEGA 10对遗传距离矩阵聚类分析,并用iTOL进行注释,利用Structure 2.3.4对甘薯种质进行群体结构分析。结果表明,对378份引进种进行分析,共扩增获得120个多态性位点,平均每对引物4个多态性位点。遗传距离矩阵结果显示种质间遗传距离最大为0.6207,遗传距离最小为0.0448,平均遗传距离为0.4027。利用MEGA10进行聚类,在遗传距离为0.2960处划分为3个类群,类群I包含29份种质、类群II包含104份种质、类群Ⅲ包含245份种质;群体结构分析表明在ΔK=2时值最大,划分为2个组群,聚类与群体结构分析结果类似。组群2中,主要以2015年以后新引进种质为主(95.4%),表明新引进种质丰富了甘薯引进种的遗传多样性,拓宽了原有资源库中的群体结构。本文通过SSR引物对378份甘薯引进种种质进行遗传多样性和群体结构分析,可为甘薯种质创新、遗传育种、优异基因挖掘利用提供参考。Sweetpotato is originated from South and Central America,and the introduction of foreign species can not only increase the total quantity amount of germplasm resources of China,but also dramatically change the breeding bottleneck of sweetpotato with narrow genetic base.In this study,378 sweetpotato germplasm collections from the national sweetpotato in vitro Genebank in Xuzhou were genotyped using 30 pairs of simple sequence repeat(SSR)primers by high-throughput nucleic acid fragment analyzer.Phylogenetic affinities among the introduced sweetpotato germplasm were analyzed to provide a theoretical basis for sweetpotato germplasm innovation and utilization.The bands were read using PROSize 3.0 software to establish the 01 database.The 01 database were imported into Darwin software to calculate Jaccard genetic distance matrix,clustered analysis of genetic distance matrix using MEGA 10,and then annotated with iTOL.Population structure of sweetpotato germplasm was constructed using Structure 2.3.4.The results showed that a total of 120 polymorphic loci were amplified in 378 sweetpotato germplasm,with an average of four polymorphic loci per primer pair.The genetic distance matrix revealed that the average genetic distance was 0.4027,the maximum genetic distance was 0.6207 and the minimum genetic distance was 0.0448.Clustering analysis of 378 sweetpotato germplasm were divided into three groups in 0.2960 genetic distance.Group I,II,and III contained 29,104,and 245 collections,respectively.Population structure analysis indicated two clades were identified base on the largest value at DK=2.Clade II(95.4%)was dominated by newly introduced germplasm after 2015,indicating that the newly introduced germplasm enriched the genetic diversity of sweetpotato collections and broadening the population structure in the original resource base.Therefore,the genetic diversity and population structure analysis of 378 introduced sweetpotato germplasm can provide a reference for sweetpotato germplasm innovation,genetic breeding,and elite a

关 键 词：甘薯引进种 遗传多样性 群体结构 

分 类 号：S531[农业科学—作物学]