老年维持性血透患者叶酸治疗与miR-150-5p血清水平的相关性研究  

Correlation between folic acid therapy and serum level of miR-150-5p in elderly patients undergoing maintenance hemodialysis

作　　者：王珊[1] 马清[1] 姚兰[1] 杨华昱[1] Shan Wang;Qing Ma;Lan Yao;Huayu Yang(Medical Care Center of Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院医疗保健中心,100050

出　　处：《中华肾病研究电子杂志》2023年第3期139-144,共6页Chinese Journal of Kidney Disease Investigation（Electronic Edition）

基　　金：国家自然科学基金资助项目(81601210);北京市医院管理局"青苗"计划(QML20170103)。

摘　　要：目的探讨老年维持性血透患者服用叶酸与微小RNA-150-5p(miR-150-5p)血清水平的关系。方法2020年1月至2021年1月于首都医科大学附属北京友谊医院医疗保健中心收治的老年维持性血液透析(MHD)患者28例,根据是否服用叶酸治疗分为用药组(12例)和对照组(16例)。比较两组的基线资料、用药前后叶酸和肿瘤标志物水平。实时定量PCR检测血清miR-150-5p水平,评估叶酸水平与miR-150-5p水平、肿瘤标志物之间的相关性。利用生物信息学和KEGG模型分析miR-150-5p可能参与的信息通路。结果与对照组相比,用药组的叶酸水平明显增高,而癌胚抗原(CEA)、癌抗原125(CA125)含量以及miR-150-5p表达量降低(P<0.05)。与用药前相比,用药后的叶酸水平显著上升(P<0.001),而miR-150-5p表达量则明显下调(P<0.05)。Pearson相关性分析提示血清叶酸与miR-150-5p呈负相关(P<0.05)。生物信息学分析提示miR-150-5p的下游靶基因多与肿瘤信号通路相关。结论老年MHD患者服用叶酸后,miR-150-5p和CEA水平均有下降。叶酸与miR-150-5p呈负相关,而miR-150-5p的下游靶基因多与肿瘤信号通路相关,提示通过叶酸调控miR-150-5p表达,有可能影响肿瘤的发生发展。Objective To investigate the relationship between folic acid intake and serum level of miR-150-5p in elderly patients undergoing maintenance hemodialysis.Methods From January 2020 to January 2021,28 elderly patients undergoing maintenance hemodialysis in the Beijing Friendship Hospital Medical Care Center were divided into a treatment group(n=12)with folic acid therapy and a control group(n=16)without folic acid therapy.The baseline data,serum folic acid and tumor markers were compared.Real-time PCR was used to analyze the serum level of miR-150-5p.The correlation among the folic acid,miR-150-5p,and tumor markers was analyzed.Bioinformatics and KEGG model were used to analyze potential regulatory pathways of miR-150-5p.Results Compared with the control group,the treatment group showed higher serum level of folic acid,but lower serum levels of CEA,CA125,and miR-150-5p(P<0.01).In comparison with those before the treatment,folic acid level was significantly increased after treatment(P<0.001),while miR-150-5p was significantly down-regulated(P<0.05).Pearson correlation analysis showed that serum folic acid level was negatively correlated with the serum level of miR-150-5p(P<0.05),and bioinformatics analysis suggested that most of the downstream target genes of miR-150-5p were involved in tumor signaling pathways.Conclusion After receiving the folic acid therapy,the elderly patients with maintenance hemodialysis had lower serum levels of miR-150-5p and CEA decreased.The serum level of folic acid was negatively correlated with miR-150-5p,while the downstream target genes of miR-150-5p were mostly involved in tumor signaling pathways,suggesting that regulating miR-150-5p expression through folic acid might have a role to affect the occurrence and development of tumors.

关 键 词：维持性血液透析 老年 叶酸 微小RNA 肿瘤生物标志物 

分 类 号：R692.5[医药卫生—泌尿科学]