人胎盘间充质干细胞治疗小鼠变应性鼻炎的有效性研究  

Study on the efficacy of human placental mesenchymal stem cells in the treatment of allergic rhinitis in mice

作　　者：韩笑 孙庆斌 杨玉娟 连培文 姚尧 杨欣 于国华[5] 张宇[1,2,3] 宋西成 HAN Xiao;SUN Qingbin;YANG Yujuan;LIAN Peiwen;YAO Yao;YANG Xin;YU Guohua;ZHANG Yu;SONG Xicheng(Department of Otolaryngology Head and Neck Surgery,Yantai Yuhuangding Hospital,Qingdao University,Yantai,Shandong,264000,China;Shandong Provincial Clinical Research Center for Otorhinolaryngologic Diseases,Yantai,Shandong,264000,China;Taishan Scholars Laboratory,Department of Central Laboratory,Qingdao University,Yantai,Shandong,264000,China;Taishan Scholars Laboratory,Qingdao University,Yantai,Shandong,264000,China;Taishan Scholars Laboratory,Department of Pathology,Yantai Yuhuangding Hospital,Qingdao University,Yantai,Shandong,264000,China)

机构地区：[1]青岛大学附属烟台毓璜顶医院耳鼻咽喉头颈外科,山东烟台264000 [2]山东省耳鼻喉疾病临床医学研究中心,山东烟台264000 [3]青岛大学附属烟台毓璜顶医院泰山学者实验室,山东烟台264000 [4]青岛大学附属烟台毓璜顶医院中心实验室,山东烟台264000 [5]青岛大学附属烟台毓璜顶医院病理科,山东烟台264000

出　　处：《中国耳鼻咽喉头颈外科》2023年第5期311-315,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金面上项目(82071021);山东省重点研发计划(重大科技创新工程)项目(2020CXGC011302)。

摘　　要：目的探讨人胎盘间充质干细胞(human placenta derived mesenchymal stem cells,HP-MSCs)对变应性鼻炎(AR)小鼠的治疗效果。方法将32只BALB/c小鼠随机平均分为4组(表示为:致敏/激发/治疗):对照组(PBS/PBS/PBS),对照+HP-MSCs组(PBS/PBS/HP-MSCs),AR+PBS组(OVA/OVA/PBS),AR+HP-MSCs组(OVA/OVA/HP-MSCs)。建立卵清蛋白(OVA)联合氢氧化铝诱导的小鼠AR模型,在造模的第20天尾静脉注射0.2 ml PBS或HP-MSCs。评估各组小鼠AR鼻部症状变化;血涂片瑞氏染色观察外周血中嗜酸性粒细胞比例;酶联免疫吸附试验(ELISA)法测定外周血Ig E、Ig G1水平及鼻黏膜白细胞介素4(IL-4)、IL-5、IL-13水平。结果AR+HP-MSCs治疗组AR组小鼠AR行为症状学评分显著减轻(6.25±0.89vs.3.38±0.74,P<0.001);外周血嗜酸性粒细胞比例明显降低[(1.63±1.03)%vs.(4.13±2.57)%,P<0.05];外周血Ig E、Ig G1水平明显降低[(36.89±2.34)ng/ml vs.(57.23±2.81)ng/ml,(21.35±0.80)ng/ml vs.(27.23±0.55)ng/ml,P均<0.05];鼻黏膜IL-4、IL-5、IL-13水平降低[(229.64±12.23)pg/mlvs.(364.17±18.81)pg/ml;(48.70±3.87)pg/mlvs.(68.36±6.88)pg/ml;(41.27±2.48)pg/ml vs.(60.60±4.75)pg/ml,P均<0.05]。结论静脉注射HP-MSCs可以有效治疗小鼠AR,为进一步治疗人AR提供新思路与理论基础。OBJECTIVE To explore the therapeutic effect of human placenta derived mesenchymal stem cells(HPMSCs)on allergic rhinitis(AR)mice.METHODS Thirty-two BALB/c mice were randomly divided into 4 groups(sensitized/challenged/treated):control group:PBS/PBS/PBS;control+HP-MSCs group:PBS/PBS/HP-MSCs;AR+PBS group:OVA/OVA/PBS;HP-MSCs group:OVA/OVA/HP-MSCs.The mouse AR model induced by ovalbumin(OVA)combined with aluminum hydroxide was established,and 0.2 ml PBS or HP-MSCs was injected into the tail vein on the 20th day of modeling.The changes of nasal symptoms in each group were evaluated.The proportion of eosinophils in peripheral blood was observed by Wright staining,and the levels of IgE and IgG1 in peripheral blood and interleukin-4(IL-4),IL-5 and IL-13 in nasal mucosa were measured by enzyme-linked immunosorbent assay(ELISA).RESULTS The behavioral symptom score of mice in AR+HP-MSCs group was significantly lower than that in AR group(6.25±0.89 vs.3.38±0.74,P<0.001).The proportion of eosinophils in peripheral blood was significantly lower than that in AR group[(1.63±1.03)%vs.(4.13±2.57)%,P<0.05].The levels of IgE and IgG1 in peripheral blood decreased significantly[(36.89±2.34)ng/ml vs.(57.23±2.81)ng/ml,(21.35±0.80)ng/ml vs.(27.23±0.55)ng/ml,P<0.05].The levels of IL-4,IL-5 and IL-13 in nasal mucosa decreased[(229.64±12.23)pg/ml vs.(364.17±18.81)pg/ml;(48.70±3.87)pg/ml vs.(68.36±6.88)pg/ml;(41.27±2.48)pg/ml vs.(60.60±4.75)pg/ml,P<0.05].CONCLUSION Intravenous injection of HPMSCs could effectively treat AR in mouse model,which provides a new idea and theoretical basis for further treatment of human AR.

关 键 词：鼻炎 过敏 干细胞 免疫调节 人胎盘间充质干细胞 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]