金线莲内生真菌Aspergillus sp.J218的次生代谢产物研究  

Secondary metabolites of endophytic fungus Aspergillus sp.J218 from Anectochilus roxburhii

作　　者：何旭辉 李祥昂 陈美言 王一丁 吴睿 郑承剑 HE Xuhui;LI Xiang-ang;CHEN Meiyan;WANG Yiding;WU Rui;ZHENG Chengjian(School of Pharmacy,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学药学系,上海200433

出　　处：《药学实践与服务》2023年第7期411-415,442,共6页Journal of Pharmaceutical Practice and Service

基　　金：国家自然科学基金资助项目(82174081);上海市浦江人才计划(21PJD082)。

摘　　要：目的研究从金线莲Anectochilus roxburhii中分离的一株曲霉属内生真菌Aspergillus sp.J218的次生代谢产物。方法利用Sephadex LH-20凝胶、硅胶和高效液相制备等色谱法从J218固体发酵产物的乙酸乙酯部位分离化合物,采用波谱方法对化合物进行结构鉴定。结果从金线莲内生真菌Aspergillus sp.J218的次生代谢产物中共分离得到10个化合物,分别鉴定为kotanin(1)、flavasperone(2)、aurasperone B(3)、fonsecinone B(4)、fonsecinone D(5)、tensidol A(6)、fonsecinone A(7)、fonsecinone C(8)、aurasperone A(9)和fonsecinone F(10)。结论从金线莲内生真菌曲霉属Aspergillus sp J218次生代谢产物分离鉴定多为二聚萘并吡喃酮类化合物,提示该菌株含有丰富的二聚化合成酶,可为后续探索该菌株中二聚萘并吡喃酮类化合物的生物合成途径提供线索。Objective To isolate the secondary metabolites of endophytic fungus Aspergillus sp.J218 from Anectochilus roxburhii.Methods Different chromatographic methods,including Sephadex LH-20 and silica gel chromatography as well as HPLC,were used to isolate compounds from the EtOAc fraction of the solid fermentation of J218,and their structures were identified by spectral methods.Results Ten compounds were isolated from the fermentation of J218 and their structures were individually identified as kotanin(1),flavasperone(2),aurasperone B(3),fonsecinone B(4),fonsecinone D(5),ensidol A(6),fonsecinone A(7),fonsecinone C(8),aurasperone A(9),and fonsecinone F(10).Conclusion Most compounds isolated from endophytic fungus Aspergillus sp.J218 in Anectochilus roxburhii were identified as dimeric naphthopyrones.These results suggest that this strain contains rich dimerization synthase,which could provide clues for the further exploration of the rational biosynthesis pathway of dimeric naphthopyrones in this strain.

关 键 词：曲霉属 金线莲 内生真菌 次生代谢产物 二聚萘并吡喃酮类化合物 

分 类 号：R915[医药卫生—微生物与生化药学]