Sequence and Bioinformatics Analysis on MSTN Gene of the Hybrid Grouper Derived from(Epinephelus fuscoguttatus×Epinephelus polyphekadion)  

作　　者：Liu CAO Pan CEHN Youhua ZHANG Xiaomei FU Hai Huang 

机构地区：[1]Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education,Sanya 572022,China [2]Yazhou Bay Innovation Institute,Sanya 572022,China [3]Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Sanya 572022,China [4]Hainan Tropical Ocean University,Sanya 572022,China

出　　处：《Agricultural Biotechnology》2023年第3期46-49,54,共5页农业生物技术（英文版）

基　　金：Supported by the Innovation Platform for Academicians of Hainan Province(YSPTZX202103);Hainan Provincial Natural Science Foundation of China(321QN263);National Natural Science Foundation of China(32160861);the Major Science and Technology Plan of Hainan Province(ZDKJ2021017);State Key Laboratory of Developmental Biology of Freshwater Fish(2020KF001);Scientific Research Foundation of Hainan Tropical Ocean University(RHDRC202010)。

摘　　要：[Objectives]This study aimed to investigate the sequence structure and function of Myostatin(MSTN)gene in the hybrid grouper(Epinephelus fuscoguttatus,♀×Epinephelus polyphekadion,♂).[Methods]Genetic DNA samples were extracted from the caudal fins of the hybrid grouper and its parents to amplify their MSTN genes.Then,MSTN gene sequences were analyzed using bioinformatics tools to predict their protein structures and functions.[Results]The hybrid grouper and its parents shared the same MSTN gene structure,consisting of three exons and two introns.Nucleotide sequence of the gene could be translated into 376 amino acids,including an N-terminal signal peptide,a proteolytic processing site(RXXR motif),and nine conserved cysteine residues at C-terminal,which were the typical features of transforming growth factor beta(TGF-β)superfamily proteins.Alignment of protein sequence showed that MSTN was highly conserved between the hybrid grouper and its parents.Especially,exon 3,an important functional domain,exhibited a sequence similarity of 100%among them.In addition,four variable amino acid residues were detected in exon 2 at positions 141,153,185 and 186 in the hybrid grouper,but they did not affect the secondary structure of the protein.[Conclusion]These results will provide molecular information for future investigation on the growth and heterosis of hybrid grouper species,and on the roles of MSTN gene in regulating the growth traits of the hybrid grouper.

关 键 词：GROUPER MSTN gene Growth traits Gene structure 

分 类 号：S917.4[农业科学—水产科学]