Effects of a Combination of Fu Zi and Rou Gui on Intestinal Neurotransmitters and Microflora in Rats with Slow Transit Constipation  

作　　者：Yuchuan LI Yuanzhe ZHANG 

机构地区：[1]The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China [2]Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

出　　处：《Agricultural Biotechnology》2023年第3期79-86,91,共9页农业生物技术（英文版）

基　　金：Supported by The Natural Science Foundation of Guizhou Province (Qiankehe Jichu[2020]1Y362);Special Project for Scientific and Technological Research on Traditional Chinese Medicine and Ethnic Medicine of Guizhou Province(QZYY-2021-016);The Natural Science Foundation of Guizhou Province(Qiankehe Jichu-ZK[2022]Yiban 510);Young Science and Technology Talents Growth Project of Guizhou Provincial Department of Education (Qian Jiao He KY Zi[2022]No. 261)。

摘　　要：[Objectives] This study was carried out to explore the combined effects of Fu Zi(Radix Aconiti Lateralis Praeparata, the secondary root of perennial herbaceous plant Acontium carmichaeli Dehx. of family Ranunculaceae) and Rou Gui(Cortex Cinnamomi, the bark of Cinnamamunz cassia Presl of family Lauraceae) on intestinal neurotransmitters and microflora in rats with slow transit constipation(STC). [Methods] Experimental rats were given loperamide hydrochloride by gavage to induce STC, and then treated with Fu Zi alone, Rou Gui alone, a combination of Fu Zi and Rou Gui(2:1 w/w), and prucalopride, respectively, for 14 days. Meanwhile, the general condition, the time to first black stool and the rate of intestinal propulsion of rats in each group were observed after STC was induced and after drug treatment, and the pathological changes in rat colon were observed via hematoxylin-eosin(HE) staining, and the levels of colonic 5-hydroxytryptamine(HT), vasoactive intestinal peptide(VIP) and substance P(SP) were detected by ELISA, and the changes in intestinal flora were detected by 16S rRNA Real-time PCR. [Results] Compared with healthy rats, the time to first black stool and the rate of intestinal propulsion, colonic 5-HT and SP levels significantly decreased(p<0.01), while their colonic VIP level significantly increased(p<0.01). Compared with STC rats, the time to first black stool, the rate of intestinal propulsion, colonic 5-HT and SP levels in Fu Zi-Rou Gui(2:1) treated rats and prucalopride treated rats significantly increased(p<0.01), while their colonic VIP level significantly decreased(p<0.01). There was no significant difference in alpha diversity between healthy rats and STC rats. However, analysis on beta diversity revealed that there were differences in microflora structure and composition between them. Compared with healthy rats, the relative abundance of Firmicutes and Proteobacteria in STC rats significantly increased, while that of Bacteroidetes decreased. Compared with STC rats, the relative abundance of P

关 键 词：Slow transit constipation(STC) Fu Zi-Rou Gui Intestinal motility NEUROTRANSMITTERS Intestinal microflora 

分 类 号：R285.5[医药卫生—中药学]