双功能型纤溶酶的研究进展  

Research Progress of Dual-Functional Fibrinolytic Enzyme

作　　者：严慧[1] 周晶晶 林宏峻 唐明青[1] YAN Hui;ZHOU Jingjing;LIN Hongjun;TANG Mingqing(School of Medicine,Huaqiao University,Quanzhou 362021,China)

机构地区：[1]华侨大学医学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2023年第4期427-434,共8页Journal of Huaqiao University(Natural Science)

基　　金：福建省科技计划项目(2019J01070);福建省泉州市科技计划项目(2018Z012);福建省厦门市科技计划项目(3502Z20227197);福建省中青年教师教育科研项目(JA14020);华侨大学人才引进项目(14BS111)。

摘　　要：综述双功能型纤溶酶(dFE)的物种来源,介绍双功能型纤溶酶的纯化工艺和酶活特性,为其进一步开发提供参考.结果表明:双功能型纤溶酶是一种同时具有纤溶酶原激活功能和纤维蛋白直接降解功能的新型纤溶物质,其来源广泛,多为微生物来源;分离纯化工艺繁琐,以多层色谱为主;大多体现丝氨酸蛋白酶活性特征,受金属离子、pH值和蛋白酶抑制剂影响较大.The species origin of dual-functional fibrinolytic enzyme(dFE)was reviewed,and the purification processes and enzyme activity characteristics of dFE were introduced to provide reference for its further development.The results showed that dFE was a novel fibrinolytic agent with both the functions of plasminogen activation and direct fibrinolysis.It had a wide range of sources,mostly microorganisms sources.The separation and purification processes were complicated,mainly based on multi-layer chromatography.It mostly exhibited the enzymatic characteristics of serine proteinase,and was greatly affected by metal ions,pH value and protease inhibitors.

关 键 词：纤溶酶原 纤维蛋白 纤溶酶 纤溶 血栓 

分 类 号：Q71[生物学—分子生物学]