假臭草来源植物内生真菌生物转化(+)-柠檬烯产物分析  

Analysis of Biotransformation Products of(+)-Limonene by Plant Endophytic Fungi From Praxelis clematidea

作　　者：杨道茂[1] YANG Daomao(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China)

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2023年第4期495-501,共7页Journal of Huaqiao University(Natural Science)

基　　金：国家级大学生科创项目(202110385039);福建省个人和团队科技员项目(2022年度);华侨大学高层次人才科研启动资金资助项目(Z15X0004)。

摘　　要：为了筛选能够生物转化(+)-柠檬烯的微生物,将7株假臭草内生真菌作为生物催化剂,在30℃,150 r·min^(-1)下发酵6 d后,添加体积分数为0.5%的(+)-柠檬烯继续转化4 d,并采用薄层层析、气相色谱-质谱联用和核磁共振技术分析产物.结果表明:各真菌生物转化(+)-柠檬烯的主要产物为(1S,2S,4R)-柠檬烯-1,2-二醇、环氧柠檬烯、(1S,2R,4R)-柠檬烯-1,2-二醇等;菌株Alternaria Nees PS08生成唯一产物(1S,2S,4R)-柠檬烯-1,2-二醇,质量浓度达到(2.46±0.16)g·L^(-1).In order to screen microorganisms of biotransformation of(+)-limonene,seven endophytic fungi from Praxelis clematidea were used as biocatalysts.After 6 days of fermentation under 30℃and 150 r·min^(-1),0.5%volume fraction of(+)-limonene was added for further 4 days,the products were analyzed by Thin Layer Chromatography,Gas Chromatography-Mass Spectrometry and Nuclear Magnetic Resonance technology.The results showed that the main products of biotransformation of(+)-limonene by various fungi were(1S,2S,4R)-limonene-1,2-diol,limonene epoxide,(1S,2R,4R)-limonene-1,2-diol,etc..The strain Alternaria Nees PS08 produced only product(1S,2S,4R)-limonene-1,2-diol,and its mass concentration reached(2.46±0.16)g·L^(-1).

关 键 词：(+)-柠檬烯 假臭草 植物内生真菌 (1S 2S 4R)-柠檬烯-1 2-二醇 

分 类 号：Q53[生物学—生物化学]