多酶级联反应的构建及其在双官能团功能化学品合成中的应用  

Construction of multi-enzyme cascade reactions and its application in the synthesis of bifunctional chemicals

作　　者：李举谋 石焜 张志钧[1] 许建和[1] 郁惠蕾[1] LI Jumou;SHI Kun;ZHANG Zhijun;XU Jianhe;YU Huilei(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物工程学报》2023年第6期2158-2189,共32页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2019YFA0905000,2021YFC2102900)。

摘　　要：利用多酶级联催化反应合成精细化学品是近年来生物催化领域的研究热点。通过构建体外多酶级联体系,可以替代传统的化学合成法,实现多种双官能团功能化学品的绿色合成。本文系统介绍了多酶级联催化反应中不同级联方式的特点及其构建策略,总结了级联反应中元件酶常用的筛选方法、NAD(P)H和ATP等辅酶的再生策略及其在多酶级联反应中的应用,并且阐述了多酶级联催化反应体系在6种双官能团功能化学品,包括ω-氨基脂肪酸、烷基内酰胺、α,ω-二元羧酸、α,ω-二胺、α,ω-二醇、ω-氨基醇合成中的应用。The synthesis of fine chemicals using multi-enzyme cascade reactions is a recent hot research topic in the field of biocatalysis.The traditional chemical synthesis methods were replaced by constructing in vitro multi-enzyme cascades,then the green synthesis of a variety of bifunctional chemicals can be achieved.This article summarizes the construction strategies of different types of multi-enzyme cascade reactions and their characteristics.In addition,the general methods for recruiting enzymes used in cascade reactions,as well as the regeneration of coenzyme such as NAD(P)H or ATP and their application in multi-enzyme cascade reactions are summarized.Finally,we illustrate the application of multi-enzyme cascades in the synthesis of six bifunctional chemicals,includingω-amino fatty acids,alkyl lactams,α,ω-dicarboxylic acids,α,ω-diamines,α,ω-diols,andω-amino alcohols.

关 键 词：多酶级联 构建策略 辅酶再生 生物催化 双官能团化学品 

分 类 号：TQ072[化学工程]