单针藻(Monoraphidium sp.)4M-18对4种抗生素的敏感性分析  

Sensitivity analysis of Monoraphidium sp.4M-18 to four antibiotics

作　　者：李畅 张云野 樊阳 孙莹 尹紫良 林宜萌 葛菁萍 平文祥[1,2] LI Chang;ZHANG Yunye;FAN Yang;SUN Ying;YIN Ziliang;LIN Yimeng;GE Jingping;PING Wenxiang(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China;Key Laboratory of Microbiology,College ofHeilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院黑龙江省普通高等学校微生物重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2023年第3期309-315,共7页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金(32071519);黑龙江省自然科学基金(LH2020C089);黑龙江省省属高等学校基本科研业务费项目(2021-KYYWF-0014);黑龙江大学2022年研究生创新科研资金(YJSCX2022-240HLJU)。

摘　　要：为探讨单针藻(Monoraphidium sp.)4M-18对抗生素的敏感性,采用血球计数板监测藻细胞密度来研究卡那霉素、四环素、氯霉素和青霉素4种抗生素对单针藻4M-18生长的影响。结果表明,不同浓度的卡那霉素、四环素和氯霉素对单针藻4M-18的生长均有不同程度的抑制作用。在液体和固体培养基中,150和100 mg·L^(-1)青霉素对单针藻4M-18的生长及藻细胞形态无影响,但是对杂菌有很好的抑制效果。本研究为单针藻4M-18利用抗生素法进行无菌化处理奠定了基础,降低了其他微生物对单针藻4M-18生物量与油脂积累的不利影响。To investigate the sensitivity of Monoraphidium sp.4M-18 to four antibiotics,algal cell density was measured by hemocytometer to confirm the effects of four antibiotics,including kanamycin,tetracycline,chloramphenicol and penicillin,on the growth of Monoraphidium sp.4M-18.The results showed that differect concentrations of kanamycin,tetracycline and chloramphenicol could inhibit the growth of Monoraphidium sp.4M-18 in varying degrees.In liquid and solid media,150 and 100 mg·L^(-1)penicillin had no effect on the growth and cell morphology of Monoraphidium sp.4M-18,but had good inhibitory effect on bacteria.This study laid the foundation for the antimicrobial treatment of Monoraphidium sp.4M-18,reducing the negative effects of other microorganisms on the biomass and lipid accumulation of Monoraphidium sp.4M-18.

关 键 词：单针藻4M-18 抗生素 敏感性 微藻 

分 类 号：Q935[生物学—微生物学]