西藏黑木耳高产菌种不同生长发育时期转录组测序分析  

Sequencing analysis of high-yielding strains of Tibet Auricularia auriculata at different growth and development stages

作　　者：薛娇 于洋 张彦龙 雷虹[1,2] 曾伟民 XUE Jiao;YU Yang;ZHANG Yanlong;LEI Hong;ZENG Weimin(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China;School of Life Sciences,Heilongjiang University,Harbin 150080,China)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2023年第3期316-324,共9页Journal of Natural Science of Heilongjiang University

基　　金：西藏自治区科技计划项目(XZ201801-GA-15)。

摘　　要：以西藏黑木耳高产菌种子实体为研究对象,为探讨西藏高产菌种子实体3个不同发育阶段的基因表达变化,利用Illumina NovaSeq6000测序平台对黑木耳子实体幼耳期、成熟期和旺盛期的样品进行转录组测序,并对其差异表达基因进行注释和分析。对黑木耳子实体9个样品的测序共获得81.93Gb Clean data,组装后得到13765个Unigene,28817个Transcript,N50长度为1954bp。GO功能富集分析表明,差异表达基因主要富集在细胞组分和催化分子功能组中。KEGG功能富集分析得出,差异表达基因在碳水化合物代谢(16%)、氨基酸代谢(15%)、脂质代谢(12%)、辅助因子和维生素的代谢(9%)有显著富集。本文筛选出多条与黑木耳子实体不同生长发育阶段有关的代谢途径,包括氨基酸代谢、碳水化合物代谢、脂质代谢以及聚糖的生物合成和代谢等,可为下一步筛选和挖掘有关黑木耳子实体生长发育的功能基因提供重要数据参考,为黑木耳品种改良提供科学依据。Taking Tibet Auricularia auriculata high-yielding fungus seed entities as the research object,the transcriptome sequencing of the fruiting bodies of Auricularia auriculata at young,mature,and vigorous stages was analyzed using the Ilumina NovaSeq 6000 sequencing platform,and differently expressed genes were annotated and analyzed.Sequencing 9 auriculata auriculata fruiting body samples yielded 81.93 Gb of Clean data.The Unigene was 13765,the Transcript was 28817,and the N50 length was 1954 bp after assembly.The differently expressed genes were mostly enriched in cell components and catalytic molecular functional categories,according to GO functional enrichment analysis.The KEGG functional enrichment analysis revealed the differently expressed genes were mostly enriched in carbohydrate metabolism(16%),amino acid metabolism(15%),lipid metabolism(12%),cofactor and vitamin metabolism(12%),and cofactor and vitamin metabolism(9%).A number of metabolic pathways related to different growth and development stages of Auricularia auriculata fungus fruiting bodies,including amino acid metabolism,carbohydrate metabolism,lipid metabolism and glycan biosynthesis and metabolism,etc.This result can provide important data reference for the next screening and mining of functional genes related to the growth and development of Auricularia auriculata fungus fruiting bodies,and provide scientific basis for the improvement of Auricularia auriculata fungus varieties.

关 键 词：黑木耳 生长发育 转录组测序 子实体 

分 类 号：Q786[生物学—分子生物学]