基于三维培养模型的辐射诱导表观遗传改变研究进展  

Research Progress of Radiation-induced Epigenetic Changes Based on Three-dimensional Culture Model

作　　者：刘雁 杜亚蓉[2] 孙坤[1] LIU Yan;DU Yarong;SUN Kun(College of Life Sciences,Northwest Normal University,Lanzhou 730070,Gansu,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,Gansu,China)

机构地区：[1]西北师范大学生命科学学院,中国甘肃兰州730070 [2]中国科学院近代物理研究所,中国甘肃兰州730000

出　　处：《生命科学研究》2023年第3期223-228,共6页Life Science Research

基　　金：甘肃省重点研发计划项目(18YF1NA051);国家自然科学基金青年科学基金项目(11705248)。

摘　　要：相比于传统的单层细胞培养,三维细胞培养(three-dimensional cell culture,TDCC)可以更准确地模拟体内细胞的生长环境,近年来被越来越多地应用于生物基础研究。表观遗传修饰与细胞癌变等许多细胞过程联系紧密。辐射是肿瘤放射治疗的主要手段之一,肿瘤放射治疗过程常常伴随着细胞表观遗传的动态变化,它会诱导机体产生包括DNA甲基化、染色质重塑、组蛋白修饰和非编码RNA调控在内的表观遗传的改变。基于TDCC模型探究辐射诱导表观遗传调控发生的机制,对于肿瘤患者的治疗和防护具有重要意义。Compared with the traditional monolayer culture,three-dimensional cell culture(TDCC)can more accurately simulate the growth environment of cells in vivo.In recent years,TDCC has been increasingly used in basic biological research.Epigenetic modification is closely linked to many cellular processes such as cell canceration.Radiation is one of the main means of tumor radiotherapy,the process of which is often accompanied by dynamic epigenetic changes,leading to DNA methylation,chromatin remodeling,histone modification and regulation of non-coding RNA.Exploring the mechanism of radiation-induced epigenetic regulation based on TDCC model is of great significance for treatment and protection of cancer patients.

关 键 词：三维细胞培养(TDCC) 表观遗传调控 电离辐射 微RNA DNA甲基化 染色质重塑 

分 类 号：Q813.11[生物学—生物工程]