BVDV与IBRV二重二温式PCR检测方法的建立及应用  

Establishment and application of a dual two-temperature PCR assay for BVDV and IBRV

作　　者：赵辉 梁晓珊 王雪妍 高瑞 谢玉杰 许立华[1] Zhao Hui;Liang Xiaoshan;Wang Xueyan;Gao Rui;Xie Yujie;Xu Lihua(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《农业科学研究》2023年第2期1-5,共5页Journal of Agricultural Sciences

基　　金：宁夏奶牛育种专项“优质高产奶牛安全健康养殖技术研究与示范”(2019NYYZ0503);宁夏回族自治区东西部科技合作项目“肉牛健康养殖技术集成与示范”(2017BY078)。

摘　　要：针对牛病毒性腹泻病毒(BVDV)的5′非翻译区(5′UTR)和牛传染性鼻气管炎病毒(IBRV)的gB基因分别选取2对引物,建立这2种病毒的二重二温式PCR检测方法,该方法特异性高,可检出BVDV与IBRV,与牛轮状病毒等均无交叉反应;对BVDV与IBRV的最低检测质量浓度为1.32、0.047 mg/L。使用该方法对采集自宁夏地区的113份具有BVDV与IBRV临床症状的牛的粪便进行检测,结果显示BVDV与IBRV的阳性检出率分别为12.39%、22.12%,2种病原体混合感染的阳性检出率为7.08%。与单重二温式PCR检测结果进行比较,得出BVDV阳性符合率为93.33%,IBRV阳性符合率为92.53%,2种病原体混合感染的阳性符合率为100%。Two pairs of primers were selected for the 5′untranslated region(5′UTR)of bovine viral diarrhea virus(BVDV)and the gB gene of infectious bovine rhinotracheitis virus(IBRV),respectively,to establish a dual twotemperature PCR assay for both viruses.This method was highly specific and could detect BVDV and IBRV without crossreactivity with bovine rotavirus,with the minimum mass concentrations of BVDV and IBRV being 1.32 mg/L and 0.047 mg/L.It was used to test 113 cattle faeces with clinical signs of BVDV and IBRV collected from Ningxia,and the results showed that the positive detection rates for BVDV,IBRV and both were 12.39%(14/113),22.12%(25/113)and 7.08%(8/113),respectively.Compared with the results of the single two-temperature PCR,the positive compliance rate was 93.33%for BVDV,92.53%for IBRV and 100%for mixed infections of both.

关 键 词：牛病毒性腹泻病毒 牛传染性鼻气管炎病毒 二重二温式PCR 检测 

分 类 号：S855.3[农业科学—临床兽医学]