银质针导热治疗肌筋膜疼痛综合征大鼠骨骼肌线粒体和SIRT3表达的变化  

Effects of silver needle-thermal conduction therapy on skeletal muscle mitochondria and silent information regulator homolog 3 expression in a rat model of myofascial pain syndrome

作　　者：王玥 张玉函 王家益 黄媛馨 沃春新 王彩霞 周沛然 王林 Wang Yue;Zhang Yuhan;Wang Jiayi;Huang Yuanxin;Wo Chunxin;Wang Caixia;Zhou Peiran;Wang Lin(Guizhou Medical University,Guiyang 550004,Guizhou Province,China;Department of Pain,Union Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China;Neuroelectrophysiology Center,Union Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州医科大学,贵州省贵阳市550004 [2]贵州医科大学附属医院疼痛科,贵州省贵阳市550004 [3]贵州医科大学附属医院电生理中心,贵州省贵阳市550004

出　　处：《中国组织工程研究》2024年第14期2202-2208,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82060811),项目负责人:王林;贵州省科技计划项目(黔科合基础-ZK[2021]),项目负责人:黄媛馨;2022年国家级大学生创新创业训练计划项目(202210660129),项目负责人:张玉函。

摘　　要：背景:临床研究发现银质针导热治疗对肌筋膜疼痛综合征患者具有良好镇痛作用,但其具体机制仍不清楚。目的:观察银质针导热治疗对肌筋膜疼痛综合征大鼠线粒体超微结构和沉默信息调节因子同源蛋白3变化的影响。方法:26只大鼠随机取20只予以打击结合运动疲劳的方法复制肌筋膜疼痛综合征大鼠模型,造模成功的16只大鼠随机分为模型组和银质针导热组,每组各8只;银质针导热组给予银质针导热处理;剩余6只为正常对照。分别于造模前1 d、造模完成后第1天、银质针导热处理后第14天检测大鼠机械刺激缩足阈值、热缩足潜伏期;银质针导热处理后第14天检测大鼠股内侧肌肌电图电活动,取大鼠右侧股内侧肌分别进行苏木精-伊红染色观察局部形态、透射电镜观察线粒体超微结构、Western blot检测沉默信息调节因子同源蛋白3表达。结果与结论:①痛阈值:与正常组和造模前相比,模型组、银质针导热组造模后机械刺激缩足阈值和热缩足潜伏期显著缩短(P<0.01);经银质针导热处理后,与模型组相比,银质针导热组机械刺激缩足阈值和热缩足潜伏期显著延长(P<0.01);②肌电图:模型组大鼠右侧股内侧出现自发电活动,银质针导热组自发电活动较模型组减少,时限较模型组延长(P<0.01),波幅较模型组降低(P<0.05);③苏木精-伊红染色:正常组大鼠肌纤维排列紧密规则,模型组大鼠肌纤维萎缩、变性,排列紊乱,银质针导热组大鼠肌肉结构紊乱改善;④骨骼肌线粒体微观结构:透射电镜显示正常组肌组织线粒体结构正常;模型组肌组织线粒体肿胀,嵴断裂或消失;银质针导热组肌组织线粒体肿胀明显缓解或趋于正常;⑤沉默信息调节因子同源蛋白3表达:模型组较正常组明显下调,银质针导热组较模型组明显上调(P<0.05);⑥结果表明:肌筋膜疼痛综合征大鼠局部肌肉线粒体出现异常,沉默信息调BACKGROUND:Clinical studies have found good analgesic effects of silver needle-thermal conduction therapy in patients with myofascial pain syndrome,but the exact mechanism remains unclear.OBJECTIVE:To observe the effect of silver needle-thermal conduction therapy on silent information regulator homolog 3(SIRT3)changes and mitochondrial ultrastructure in a rat model of myofascial pain syndrome.METHODS:Twenty rats were randomly selected from 26 Sprague-Dawley rats and were subjected to percussion combined with motor fatigue for replicating the rat model of myofascial pain syndrome.Sixteen rats that were successfully modeled were randomly divided into model group and silver needle-thermal conduction therapy group(treatment group),with eight rats in each group.The remaining rats were used as controls(normal group).The treatment group was treated with silver needle-thermal conduction therapy.Mechanical withdrawal threshold and thermal withdrawal latency of rats were measured at 1 day before modeling,1 day after modeling and 14 days after treatment.Electromyographic activities of the right medial femoral muscle were measured at 14 days after treatment.The right medial femoral muscle tissue was taken for hematoxylin-eosin staining to observe the local morphology and for transmission electron microscopy to observe the mitochondrial ultrastructure.Western blot assay was performed to detect SIRT3 expression.RESULTS AND CONCLUSION:Pain threshold:The mechanical withdrawal threshold and thermal withdrawal latency of the model and treatment groups were significantly decreased compared with those in the normal group and before modeling(P<0.01).After treatment,the mechanical withdrawal threshold and thermal withdrawal latency of rats were significantly higher in the treatment group compared with the model group(P<0.01).Electromyography:The rats in the model group showed spontaneous electrical activity in the right medial femur,while the rats in the treatment group showed reduced spontaneous electrical activity,longer time frame(

关 键 词：肌筋膜疼痛综合征 银质针导热治疗 肌肉线粒体 SIRT3 肌筋膜激痛点 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446