美国红枫‘秋火焰’组培初代筛选及增殖培养技术优化  

Optimization of the Initial Screening and Proliferation Culture Technology for Tissue Culture of the American Red Maple‘Autumn Blaze’

作　　者：王宏霞 WANG Hongxia(Heilongjiang Daqing Oilfield Ecological Environment Management Company,Daqing 163411,China)

机构地区：[1]大庆油田生态环境管护公司,黑龙江大庆163411

出　　处：《黑龙江农业科学》2023年第7期75-80,共6页Heilongjiang Agricultural Sciences

摘　　要：为促进耐寒美国红枫工厂化繁殖,试验采用引种驯化10年的耐寒美国红枫“秋火焰”(Acer rubrum‘Autumn Blaze’),探索植物生长调节剂配比、抑菌剂处理、培养基pH、采集时间对初代培养过程腋芽诱导的影响,以及茎段接种方式及不同光照强度对增殖培养的影响,并利用腋芽茎段外植体进行初代和继代增殖培养。结果表明,适宜初代培养的培养基为改良MS+6-BA 1.0 mg·L^(-1)+IBA 0.2 mg·L^(-1);在浓度为20 mg·L^(-1)的益培灵处理下,腋芽茎节生长更为适宜。以改良后的MS培养基在pH为6.0时最适宜;在6月初进行外植体的采集时美国红枫增殖系数最大,长势较好;在继代增殖时采用切茎段接种方式处理最佳,美国红枫在1600 lx光照强度下的LED光增殖效果最佳,生长速度快。In order to promote the industrialized propagation of cold-resistant American red maple,a ten-year domesticated cold-resistant Acer rubrum Autumn Blaze was used in the experiment to explore the effects of plant growth regulators,bacteriostatic agents,medium pH,and collection time on the induction of axillary buds in the primary culture process,as well as the effects of stem segment inoculation methods and different light intensities on proliferation culture.The primary and subculture proliferation culture were carried out using axillary bud stem explants.The results showed that the suitable medium for primary culture was modified MS+6-BA 1.0 mg·L^(-1)+IBA 0.2 mg·L^(-1);Under the treatment of 20 mg·L^(-1)Yipeiling,the growth of axillary bud stem nodes was more suitable.The modified MS medium was most suitable at pH 6.0;the American red maple had the highest proliferation coefficient and better growth when the explants were collected in early June;The stem cutting method was the best for subculture proliferation,and the American red maple had the best LED light proliferation effect at a light intensity of 1600 lx,with a fast growth rate.

关 键 词：美国红枫 “秋火焰” 初代培养 增殖培养 

分 类 号：S687[农业科学—观赏园艺]