气相色谱法测定闽南产罗勒挥发油中草蒿脑的含量  

Determination of Estragole in the Volatile Oil of Ocimum Basilicum in Southern Fujian by Gas Chromatography

作　　者：刘舜慧[1,2] 杨婷 张怡评 王琼珺 林泽燕[1] LIU Shun-hui;YANG Ting;ZHANG Yi-ping;WANG Qiong-jun;LIN Ze-yan(Zhangzhou Health Vocational College,Zhangzhou 363000,China;The Thrid Institute of Oceannography Ministry of Natural Resources/Technology Innovation Center for Exploitation of Marine Biological Resources,Xiamen 361005,China)

机构地区：[1]漳州卫生职业学院,福建漳州363000 [2]自然资源部第三海洋研究所/海洋生物资源开发利用工程技术创新中心,福建厦门351005

出　　处：《江西中医药大学学报》2023年第3期72-75,共4页Journal of Jiangxi University of Chinese Medicine

基　　金：福建省教育厅中青年教师教育科研项目(JAT201400);漳州卫生职业学院科研项目(ZWYZ202007);漳州卫生职业学院访问学者项目(漳卫职人[2022]1号)。

摘　　要：目的:建立测定闽南产罗勒挥发油中草蒿脑含量的方法。方法:采用气相色谱法,色谱柱为HP-5毛细管柱(30 m×320μm×0.25μm),程序升温,氢离子火焰检测器,检测器温度为270℃,进样口温度为250℃,载气为高纯氮气,流速为1 mL/min,进样量为1μL,进样分流比为10:1。结果:草蒿脑在0.1092~2.6208 mg/mL浓度范围内呈良好的线性关系(r=0.9995),定量限为0.00125 mg/mL(S/N=10)、检测限为0.00042 mg/mL(S/N=3);精密度、稳定性、重复性的RSD均小于3%;平均回收率为100.32%(RSD=2.70%,n=18)。结论:本方法操作简便、准确,精密度、重复性良好,可用于测定闽南产罗勒挥发油中草蒿脑的含量,并可作为闽南产罗勒的质量综合评价及提取工艺研究提供参考。Objective:To establish a method for determination of estragole in the volatile oil of Ocimum basilicum in southern Fujian.Method:By using GC,the chromatography was performed on an HP-5 capillary column(30 m×320μm×0.25μm)with hydrogen ion flame detector at 270℃and injection port at 250℃.The carrier gas was high purity nitrogen,and the flow rate was 1mL/min.The injection volume was 1μL and the injection split ratio was 10∶1.Results:The linear range of estragole was 0.1092~2.6208mg/mL(r=0.9995).The limit of quantitation was 0.00125 mg/mL(S/N=10)and the limit of detection was 0.00042 mg/mL(S/N=3).The RSD of precision,stability and repeatability was less than 3%.The average recovery was 100.32%(RSD=2.70%,n=18).Conclusion:The method is simple,accurate,precise and reproducible.It can be used to determine the content of estragole in the volatile oil of Ocimum basilicum in southern Fujian,and can be used as a reference for the comprehensive evaluation of the quality of Ocimum basilicum in southern Fujian and the study of extraction process.

关 键 词：罗勒 挥发油 草蒿脑 含量测定 气相色谱法 

分 类 号：R284[医药卫生—中药学]