鸭源新型鹅细小病毒YJDS-19-16株的分离鉴定及VP3基因序列分析  

Isolation and Identification of a Duck-origin Novel Goose Parvovirus Strain YJDS-19-16 and Analysis on Its VP3 Gene Sequence

作　　者：何信群 苏敬良[2] 徐玉 李来旭 李阁 刘哲君 李小意 徐光菊 田燕 王灵强 张晏 何虹霞 明月月 He Xinqun;Su Jingliang;Xu Yu;Li Laixu;Li Ge;Liu Zhejun;Li Xiaoyi;Xu Guangju;Tian Yan;Wang Lingqiang;Zhang Yan;He Hongxia;Ming Yueyue(Chongqing Health Forever Biotechnology Co.,Ltd.,Chongqing 402460,China;College of Veterinary Medicine,China Agricultural University,Beijing 100083,China)

机构地区：[1]重庆永健生物技术有限责任公司,重庆402460 [2]中国农业大学动物医学院,北京100083

出　　处：《中国动物检疫》2023年第7期17-23,共7页China Animal Health Inspection

摘　　要：为了解鸭源新型鹅细小病毒(NGPV)的生物学特性及遗传进化情况,将江苏省某鸭场送检的疑似发生鸭短喙侏儒综合征的鸭心脏、肝脏和脾脏等组织样品,经接种鸭胚分离病毒,对分离株进行ELD50测定、动物回归试验,并对其VP3基因进行遗传进化分析。分离鉴定结果显示:成功从送检的约17日龄樱桃谷鸭组织样品中分离到1株NGPV,将其命名为YJDS-19-16株;分离株YJDS-19-16 ELD50为10^(-5.7)/0.2mL,可引起试验鸭出现食欲不振、精神沉郁、采食量减少、生长发育不良和跛行等典型症状,并可成功复制出舌外露症状。构建的VP3基因遗传进化树显示,水禽细小病毒主要分为两个大支,经典番鸭细小病毒(MDPV)类为一支,经典鹅细小病毒(GPV)类为一支,且NGPV与GPV亲缘关系最近;VP3基因同源性分析结果显示:YJDS-19-16株与其他NGPV同源性为98.6%~99.9%,与经典GPV同源性为92.7%-96.1%;VP3蛋白氨基酸替换分析结果显示:第504位(S→R)为YJDS-19-16株独有。基因重组分析结果显示,YJDS-19-16株潜在的主要亲本和次要亲本分别为GPV 06-0329株和GDaGPV株,且重组可能分布于522~1060 bp。结果表明,引起鸭发生短喙侏儒综合征的分离株YJDS-19-16为鸭源NGPV,可能是一种重组病毒。本试验可丰富NGPV的研究资料,为水禽细小病毒遗传以及该病的诊断和预防等相关研究提供参考。In order to investigate the biological characteristics and genetic evolution of a duck-origin novel goose parvovirus(NGPV),hearts,livers and spleens of ducks suspected of short beak and dwarfism syndrome(SBDS)were collected and inoculated into duck embryos to isolate the virus,followed by ELDso determination of the isolate,animal regression test,and genetic evolution analysis on the VP3 gene.The results showed that one strain of NGPV was isolated from tissue samples collected from Cherry Valley ducks at about 17 days old submitted by a farm in Jiangsu Province,and named as YJDS-19-16.The ELDso of YJDS-19-16 strain was 10^(-5.7)/0.2 mL,causing typical symptoms such as inappetence,depression,decreased feed intake,stunted growth and lameness,and reproducing the symptom of tongue exposure.The genetic evolutionary tree of VP3 gene showed that,waterfowl parvovirus was mainly divided into classical muscovy duck parvovirus(MDPV)and classical goose parvovirus(GPV),and NGPV was genetically closest to GPV.The results of homology analysis on VP3 gene showed that,JDS-19-16 was 98.6%-99.9%identity to other NGPV,and 92.7%-96.1%to classical GPV.The results of amino acid substitution analysis on VP3 protein showed that the rank of 504th(S→R)was unique to YJDS-19-16 strain.Gene recombinant analysis results showed that the potential major and minor parents of YJDS-19-16 were GPV 06-0329 and GDaGPV,respectively,and the recombination might be distributed within 522-1060 bp.In conclusion,YJDS-19-16 strain causing SBDS was duckorigin NGPV,and might be a recombinant virus.Research data about NGPV was enriched by the study,providing a reference for relevant researches on the genetics,diagnosis and prevention of waterfowl parvovirus.

关 键 词：新型鹅细小病毒 短喙侏儒综合征 分离鉴定 VP3基因 序列分析 

分 类 号：S852.6[农业科学—基础兽医学]