胶原诱导性关节炎小鼠升主动脉弹性变化及与血清炎性因子的关系  

Evaluation of ascending aortic elasticity in collagen-induced arthritis mice and its relationship with serum inflammatory cytokines

作　　者：苏瑞娟[1] 梁春蕊 马宁[2] SU Rui-juan;LIANG Chun-rui;MA Ning(Department of Ultrasound,Beijing Jishuitan Hospital,Beijing 100096,China;Department of Echocardiography Beijing Children's Hospital,Capital Medical University,Beijing 100045,China)

机构地区：[1]北京积水潭医院超声科,北京100096 [2]首都医科大学附属北京儿童医院心脏超声科,北京100045

出　　处：《中华实用诊断与治疗杂志》2023年第5期444-447,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81601518);北京市自然科学基金(7192062)。

摘　　要：目的 观察胶原诱导性关节炎(collagen-induced arthritis, CIA)小鼠升主动脉组织病理、超声弹性参数及血清炎性因子水平变化,探讨CIA小鼠升主动脉超声弹性参数与血清炎性因子的相关性。方法 雄性DBA/1小鼠7只为CIA组,制备CIA模型;野生型雄性C57BL/6小鼠7只为对照组。造模第56天,测量2组小鼠收缩压、舒张压;行超声检查测量收缩期和舒张期升主动脉内径,计算升主动脉超声弹性参数包括升主动脉应变率、膨胀系数、僵硬指数。然后处死小鼠左心室取血,采用ELISA法检测血清白细胞介素-1β、肿瘤坏死因子-α、类风湿因子水平;取升主动脉组织采用HE染色和van Gieson染色观察主动脉管壁病理变化。采用Pearson相关法分析CIA小鼠升主动脉超声弹性参数与血压、血清炎性因子水平的相关性。结果 (1)CIA组舒张压[(54.50±4.79)mm Hg]、升主动脉应变率[(11.66±2.68)%]、膨胀系数[(0.91±0.15)×10^(-6)cm^(2)/dyn]均低于对照组[(59.47±3.33)mm Hg、(14.90±2.31)%、(1.20±0.26)×10^(-6)cm^(2)/dyn](P<0.05),收缩压[(99.55±3.82)mm Hg]、僵硬指数(6.08±1.99)及血清白细胞介素-1β[(15.62±1.57)ng/L]、肿瘤坏死因子-α[(28.16±6.10)ng/L]、类风湿因子[(109.36±12.23)u/mL]水平均高于对照组[(94.56±2.49)mm Hg、3.38±1.02、(3.42±1.3)ng/L、(6.40±2.65)ng/L、(12.61±2.98)u/mL](P<0.05),收缩期升主动脉内径[(1.41±0.14)mm]、舒张期升主动脉内径[(1.25±0.09)mm]与对照组[(1.51±0.11)、(1.34±0.09)mm]比较差异均无统计学意义(P>0.05)。(2)HE染色结果显示,对照组和CIA组升主动脉管壁组织结构均未见明显异常。van Gieson染色结果显示,对照组升主动脉管壁弹力纤维排列紧致、连续、着色清晰;CIA组升主动脉管壁弹力纤维排列松散、水肿、着色变淡。(3)CIA小鼠升主动脉应变率与收缩压、血清类风湿因子水平均呈负相关(r=-0.807,P<0.001;r=-0.639,P=0.014);膨胀系数与收缩压、血清类风湿因子Objective To observe the changes of the histopathology,elastic parameters and serum inflammatory cytokines of ascending aorta in mice with collagen-induced arthritis(CIA),and to explore the correlation between the elasticity of ascending aorta and serum inflammatory cytokines in CIA mice.Methods CIA model was induced by collagen in 7 male DBA/1 mice(CIA group),and another 7 male wild C57BL/6 mice were as controls(control group).The systolic blood pressure(SBP)and diastolic blood pressure(DBP)were measured 56 days after modeling.The systolic and diastolic ascending aortic diameters were measured by echocardiography,the elastic parameters as strain rate,distensibility and stiffness index were calculated.The mice were sacrificed to obtain blood from the left ventricle.The serum interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and rheumatoid factor level were detected.The pathological changes of aortic wall were observed by HE and van Gieson staining.Pearson correlation method was used to analyze the correlations of ascending aortic elasticity with blood pressure and serum inflammatory cytokines.Results(1)The DBP,strain rate of ascending aorta and distensibility were lower in CIA group[(54.5±4.79)mm Hg,(11.66±2.68)%,(0.91±0.15)×10^(-6)cm^(2)/dyn]than those in control group[(59.47±3.33)mm Hg,(14.9±2.31)%,(1.20±0.26)×10^(-6)cm^(2)/dyn](P<0.05),the SBP,stiffness index,IL-1β,TNF-αand rheumatoid factor were higher in CIA group[(99.55±3.82)mm Hg,6.08±1.99,(15.62±1.57)ng/L,(28.16±6.10)ng/L,(109.36±12.23)u/mL]than those in control group[(94.56±2.49)mm Hg,3.38±1.02,(3.42±1.3)ng/L,(6.40±2.65)ng/L,(12.61±2.98)u/mL](P<0.05),and there were no significant differences in the systolic and diastolic ascending aortic diameters between CIA group[(1.41±0.14),(1.25±0.09)mm]and control group[(1.51±0.11),(1.34±0.09)mm](P>0.05).(2)HE staining results showed no obvious abnormality in the structure of aortic wall in two groups,van Gieson staining results showed a compact arrangement,continuous and clear coloring

关 键 词：类风湿关节炎 胶原诱导性关节炎 升主动脉应变率 膨胀系数 僵硬指数 炎性因子 小鼠 

分 类 号：R593.22[医药卫生—内科学]