小麦株高相关基因TaGAMyb-B在水稻中的功能研究  

Functional Study of TaGAMyb-B Conferring Wheat Plant Height in Transgenic Rice

作　　者：刘娟 冯玉梅 韩冰 邢燕平 李淑芬 杨燕 LIU Juan;FENG Yumei;HAN Bing;XING Yanping;LI Shufen;YANG Yan(Functional Laboratory of Plant Biotechnology,College of Life Science,Inner Mongolia Agricultural University,Hohhot 010010,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学生命科学学院植物生物技术功能实验室,内蒙古呼和浩特010010 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《华北农学报》2023年第3期42-48,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31960424);饲用作物及有益微生物种质资源创新与分子育种团队(TD202103)。

摘　　要：为进一步探究小麦的TaGAMyb-B不同等位变异基因对茎秆伸长的作用,利用水稻的农杆菌转化体系、RT-qPCR、组织切片和细胞组织特异性分析等方法系统研究TaGAMyb-B基因不同等位变异中84 bp InDel的功能。结果发现:在TaGAMyb-B超表达的水稻转基因株系中,该基因在种子、根、茎以及叶中均有表达;转TaGAMyb-Ba-GFP和TaGAMyb-Bb-GFP基因型水稻T_(2)种子在应对外界NaCl、GA和甘露醇胁迫处理时,其表现的敏感性为TaGAMyb-Bb-GFP大于TaGAMyb-Ba-GFP;转TaGAMyb-Bb-GFP基因型水稻的第一、二和三茎粗,穗长和分蘖数均显著小于转TaGAMyb-Ba-GFP基因型;转基因水稻后代第二茎间的细胞组织切片分析表明:转TaGAMyb-Ba-GFP基因型水稻横切面厚壁组织细胞的平均厚度显著大于转TaGAMyb-Bb-GFP基因型水稻,并且其厚壁细胞的平均长度极显著小于转TaGAMyb-Bb-GFP基因型水稻。以上结果表明,TaGAMyb-B不同等位变异中84 bp序列缺失在转基因水稻中除了增加非生物胁迫抗性和抗倒伏性功能外,还显著增加了穗长和分蘖数。To further investigated the effect of different allelic variants of TaGAMyb-B genes in wheat on stem elongation,used the rice Agrobacterium transformation system,RT-qPCR,tissue section and cell tissue specific analysis to systematically study the function of the 84 bp InDel of TaGAMyb-B.The results showed that,in the over-expressed transgenic rice lines,transcript expression of TaGAMyb-B was detected in seeds,roots,stems and leaves;TaGAMyb-Bb-GFP transgenic seeds were more sensitive under the treatment of NaCl,GA and mannitol compared with TaGAMyb-Ba-GFP;the diameters of first,second and third stem inter-nodes,spike length and tiller number of TaGAMyb-Ba-GFP were significantly larger than that of TaGAMyb-Bb-GFP;and the analysis of cell tissue sections showed that the average thickness of the thick-walled tissue cells in the transverse sections of the transgenic TaGAMyb-Ba-GFP rice was significantly greater than that of the transgenic TaGAMyb-Bb-GFP event,the average length of thick-walled cells was extremely significantly shorter than that of transgenic TaGAMyb-Bb-GFP event.The above mentioned results indicated that the 84 bp deletion in TaGAMyb-B increased not only abiotic stress resistance and plant lodging resistance,but also spike length and tiller number in transgenic rice.

关 键 词：转基因水稻 TaGAMyb-B 等位变异 非生物胁迫 抗倒伏性 

分 类 号：S511.03[农业科学—作物学] Q78[生物学—分子生物学]