结球甘蓝遗传图谱的构建及抗黑腐病QTL定位  

Construction of a Genetic Linkage Map and QTL Mapping for Black Rot Resistance in Cabbage

作　　者：乐祥庆 钟雄辉 崔建 韩睿 宋旭朦 颉建明 康俊根[2] YUE Xiangqing;ZHONG Xionghui;CUI Jian;HAN Rui;SONG Xumeng;XIE Jianming;KANG Jungen(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China;Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Horticultural Crop Biology and Germplasm Creation in North China,Ministry of Agriculture and Rural Affairs,Beijing 100097,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070 [2]北京市农林科学院蔬菜研究所,农业农村部华北地区园艺作物生物学与种质创制重点实验室,北京100097

出　　处：《华北农学报》2023年第3期167-175,共9页Acta Agriculturae Boreali-Sinica

基　　金：国家重点研发计划项目(2022YFD1200502);国家自然科学基金项目(31972408);北京市农林科学院创新能力建设专项(KJCX20210425,KJCX20200410)。

摘　　要：为了进一步明确结球甘蓝抗黑腐病性状的遗传基础,选育优质抗病品种,以结球甘蓝高抗黑腐病1号生理小种材料4674为父本,高感材料4673为母本,杂交获得F_(1),F_(1)自交获得152个单株的F_(2)群体。采用苗期喷雾法对F_(2)群体进行接病,12~14 d后,按照甘蓝苗期鉴定方法对F_(2)群体进行表型鉴定。从404个分子标记中筛选得到175个多态性好且条带清晰的标记。随后,使用这175个分子标记对F_(2)群体进行基因型检测和遗传连锁图谱的构建。最后,结合抗病性表型鉴定数据和遗传图谱对甘蓝抗黑腐病性状的QTL进行标记定位。结果表明:有154对分子标记连锁到9条染色体上,其中包括110对InDel标记和44对SSR标记,覆盖长度714.29 cM,标记间平均图距4.64 cM。共定位到7个QTL位点,其中有3个为主效位点,分别是qBR-7-2、qBR-7-3和qBR-4-3,位于7号染色体的CG842110~CG842482及M29~M39标记间和4号染色体上的CD838151~BOE417,LOD值分别为5.75,3.20,3.47,可解释的表型贡献率分别为16.0%,9.2%,10.0%。In order to further definite the genetic basis of black rot resistance in cabbage,and breed high-quality disease resistant cultivars,F1 progenies were obtained by crossing the inbred line 4674(high resistance to race 1)as male parent,with the inbred line 4673(high susceptibility to race 1)as female parent.The F2 population containing 152 individuals were obtained by F1 self-crossing.The F2 population was inoculated by spraying at seedling stage.After 12-14 days,the phenotype of F2 population was identified according to the identification method of cabbage seedling stage.A total of 175 markers with good polymorphism and clear bands were selected from 404 pairs.Subsequently,the 175 molecular markers were used for genotyping of F2 population and constructing a genetic linkage map.Finally,the QTL for black rot resistance of cabbage was mapped with phenotypic data and genetic map.The results showed that there were 154 molecular markers linked to nine chromosomes,including 110 pairs of InDel markers and 44 pairs of SSR markers and covering a length of 714.29 cM,and the average distance between markers was 4.64 cM.Seven QTLs were located,of which three major QTLs were qBR-7-2,qBR-7-3 and qBR-4-3,mapped on the genomic markers(CG842110-CG842482 and M29-M39)of chromosome 7,and on the genomic markers between CD838151 and BOE417 of chromosome 4,respectively.The explainable phenotypic variation of QTLs were 16.0%,9.2%and 10.0%,and their LOD values were 5.75,3.20 and 3.47,respectively.

关 键 词：结球甘蓝 黑腐病 F_(2)群体 遗传连锁图谱 QTL定位 

分 类 号：S635.1[农业科学—蔬菜学]