草铵膦胁迫下小麦叶片转录组分析  

Comparative Transcriptome Analysis of Wheat Leaf in Response to Glufosinate-ammonium Stress

作　　者：于金萍 张惟 李琦 白鹏华 崔新仪[1] 刘亦学[2] YU Jinping;ZHANG Wei;LI Qi;BAI Penghua;CUI Xinyi;LIU Yixue(Tianjin Agricultural University,Tianjin 300384,China;Institute of Plant Protection,Tianjin Academy of Agriculture Sciences,Tianjin 300381,China)

机构地区：[1]天津农学院,天津300384 [2]天津市农业科学院植物保护研究所,天津300381

出　　处：《华北农学报》2023年第3期184-190,共7页Acta Agriculturae Boreali-Sinica

基　　金：天津市科技支撑项目(19YFZCSN00110)。

摘　　要：为了挖掘小麦响应草铵膦胁迫下的关键基因,解析关键基因及其家族成员染色体分布、表达模式等,以冬小麦津农6号为研究对象,喷施不同浓度的草铵膦,观察其药后表型;提取胁迫处理0,3,9 h的小麦叶片总RNA,进行转录组测序,筛选关键基因并对其表达量、结构及表达模式进行分析。结果表明:转录组分析发现草铵膦胁迫诱导TraesCS4A02G044000基因上调表达,序列分析表明,该基因位于小麦第4同源基因A基因组。蛋白结构域分析可知,该基因编码蛋白存在1个信号肽、1个DUF568结构域和1个b561结构域。此外,在该基因家族成员中挖掘出12个同时含有DUF568和b561结构域的基因,位于第4,5,7同源群,其中位于第4同源群A、B、D染色体的TraesCS4D02G265000、TraesCS4B02G265100和TraesCS4A02G044000基因在草铵膦胁迫诱导下显著上调表达,该3个基因均编码细胞色素b561。编码细胞色素b561的TraesCS4A02G044000及其同源基因在草铵膦胁迫下显著上调,表明b561在小麦受到草铵膦胁迫后产生了积极的响应,参与了草铵膦在小麦体内的代谢。In order to excavate the key genes in wheat which were resistant to glufosinate-ammonium stress and analyze family members chromosome distribution and expression pattern.Winter wheat Jinnong 6 was selected as the research object and different concentrations of glufosinate-ammonium was used in our study and observed symptoms of wheat after treatments.Total RNA was extracted from wheat leaves at 0,3 and 9 h after treatment.Transcriptomic sequencing technology was used to analyze and excavate the key genes in wheat.To confirm genes expression,protein structure of their family members and expression pattern.The results showed that TraesCS4A02G044000 was up-regulated induced by glufosinate-ammonium stress according to transcriptome analysis.Sequence analysis showed that TraesCS4A02G044000 was located in the 4th homologous gene A genome of wheat.The protein domain analysis showed that the protein encoded of TraesCS4A02G044000 had a signal peptide,a DUF568 and a b561.Twelve genes containing DUF568 and b561 domains were excavated from the genome family members,which were located in the 4th,5th and 7th homologous groups.The expression pattern analysis showed that TraesCS4D02G265000,TraesCS4B02G265100 and TraesCS4A02G044000 located in chromosomes A,B and D of the 4th homologous group which encoded cytochrome b561 were up-regulated obviously in wheat under glufosinate-ammonium stress.TraesCS4A02G044000 and its homologous genes encoding cytochrome b561 were significantly up-regulated under glufosinate-ammonium stress,indicated that b561 had a positive response to glufosinate-ammonium stress and participated in the metabolism of glufosinate-ammonium in wheat.

关 键 词：小麦 草铵膦胁迫 转录组分析 基因定位及表达模式 

分 类 号：S512[农业科学—作物学]