Claudin-1,3在寻常型银屑病患者血清中的表达及临床意义  

Expression and clinical significance of Claudin-1 and Claudin-3 in the serum of patients with psoriasis vulgaris

作　　者：马今朝 顿耿 于子红 王哲新 靳旭 MA Jinzhao;DUN Geng;YU Zihong;WANG Zhexin;JIN Xu(Kaifeng People's Hospital,,Kaifeng 475000,China;Kaifeng Cambien Laboratory of Medical Laboratory,Kaifeng 475000,China)

机构地区：[1]开封市人民医院,河南开封475000 [2]开封康必恩医学检验实验室,河南开封475000

出　　处：《皮肤性病诊疗学杂志》2023年第3期205-210,共6页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：开封市科技发展计划项目(2107012)。

摘　　要：目的探讨Claudin-1,3在寻常型银屑病(PV)患者血清中的表达及临床意义。方法选取122例PV患者(包括轻度组36例、中度组54例、重度组32例)及60例健康体检者(对照组),采用ELISA检测血清超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)、Claudin-1,3水平;采用logistics回归分析血清Claudin-1,3与PV的关系,ROC曲线分析其诊断价值。结果PV组的hs-CRP、TNF-α及IL-6水平均明显高于对照组(均P<0.05),Claudin-1水平明显低于对照组(t=11.96,P<0.001),Claudin-3水平明显高于对照组(t=10.64,P<0.001)。PV患者中,重度组血清Claudin-1水平明显低于轻、中度组,中度组血清Claudin-1水平明显低于轻度组(均P<0.05)。PV患者Claudin-1水平与病情严重程度(r=-0.38)、hs-CRP(r=-0.18)及TNF-α(r=-0.22)均呈负相关(均P<0.05)。重度组血清Claudin-3水平明显高于轻、中度组,中度组血清Claudin-3水平明显高于轻度组(均P<0.05)。PV患者血清Claudin-3水平与病情严重程度(r=0.41)、hs-CRP(r=0.20)、TNF-α(r=0.18)及IL-6(r=0.36)呈正相关(均P<0.05)。血清Claudin-1与Claudin-3水平呈负相关(r=-0.22,P=0.015),二者诊断PV的曲线下面积分别为0.875和0.867,明显高于TNF-α(Z值分别为4.16、3.60,均P<0.001)和IL-6(Z值分别为3.63、3.52,均P<0.001)。结论PV患者血清中的Claudin-1,3异常表达。Claudin-1,3与病情严重程度及炎症指标有关,是PV发病的独立危险因素,对PV具有一定的诊断价值。Objective To investigate the expression and clinical significance of serum Claudin-1 and Claudin-3 levels in patients with psoriasis vulgaris(PV).Methods A total of 122 patients with PV were enrolled as the PV group(including 36 cases in the mild group,54 cases in the moderate group,32 cases in the severe group),and 60 healthy controls were enrolled from the physical examination center.The serum levels of hypersensitive C-reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin6(IL-6),Claudin-1 and Claudin-3 were measured using enzyme linked immunosorbent assay(ELISA).Logistic regression was used to analyze the relationship between serum levels of Claudin-1 and Claudin-3 and PV severity,and the receiver operating characteristic(ROC)curve was used to analyze its diagnostic value.Results The levels of hs-CRP,TNF-αand IL-6 in PV group were significantly higher than that in the controls(P<0.05).The serum levels of Claudin-1 in the PV group were significantly lower than that in the controls(t=11.96,P<0.001),while the serum levels of Claudin-3 was significantly higher in the PV group(t=10.64,P<0.001).The serum levels of Claudin-1 were negatively correlated with disease severity(r=-0.38),hs-CRP(r=-0.18)and TNF-αlevelsm(r=-0.22)(all P<0.05).In contrast,serum levels of Claudin-3 were positively correlated with disease severity(r=0.41),and serum levels of hs-CRP(r=0.20),TNF-α(r=0.18)and IL-6(r=0.36)(all P<0.05).Moreover,serum levels of Claudin-1 were negatively correlated with Claudin-3(r=-0.22,P=0.015).The areas under the curve of Claudin-1 and Claudin-3 for the diagnosis of PV were 0.875 and 0.867,respectively,which were significantly higher than that of TNF-α(Z values were 4.16 and 3.60,respectively,P<0.001)and IL-6(Z values were 3.63,3.52,respectively,P<0.001).Conclusions The altered expression levels of serum Claudin-1 and Claudin-3 in PV patients are associated with disease severity and the levels of inflammatory markers.Alterations of Claudin-1 and Claudin-3 expression are independent risk factors f

关 键 词：CLAUDIN-1 CLAUDIN-3 寻常型银屑病 病情严重程度 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]