基于指纹图谱结合化学计量学的独活寄生丸的质量评价研究  

Quality evaluation of Duhuo Jisheng Pills based on fingerprint combined with stoichiometry

作　　者：张倩倩 卓雪群 孙淑慧 张筠昊 梁霄 杨婧[1] 王锐[1] ZHANG Qian-qian;ZHUO Xue-qun;SUN Shu-hui;ZHANG Jun-hao;LIANG Xiao;YANG Jing;WANG Rui(Heilongjiang University of Traditional Chinese Medicine,Harbin 150006,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150006

出　　处：《现代药物与临床》2023年第6期1367-1373,共7页Drugs & Clinic

基　　金：国家自然科学基金资助项目(82074271);中央支持地方高校改革发展资金(2020YQ05)。

摘　　要：目的建立独活寄生丸的指纹图谱,并采用化学计量学进行评价。方法采用HPLC法建立独活寄生丸的指纹图谱和相似度评价,以聚类分析、主成分分析、正交偏最小二乘判别分析法分析数据。结果建立了独活寄生丸HPLC特征指纹图谱,共确定32个共有峰,指认6个成分龙胆苦苷、川芎嗪、芝麻脂素、蛇床子素、细辛脂素、二氢欧山芹醇当归酸酯。15批独活寄生丸与对照图谱的相似度均大于0.900。15批独活寄生丸样品可分为2类,S1、S4、S6聚为一类,其余样品为另一类。9个主成分因子可作为独活寄生丸的评价指标。对模型分类贡献较大的3个变量,分别为共有峰4、17、2。结论该方法可用于独活寄生丸的质量评价,为独活寄生丸的质量标准研究提供依据。Objective To establish the fingerprint of Duhuo Jisheng Pills,and to evaluate it with stoichiometry.Methods Fingerprints of Duhuo Jisheng Pills were established by HPLC method,and similarity was evaluated.Cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis were used to analyze the data.Results The characteristic fingerprint of Duhuo Jisheng Pills was established and 32 common peaks were established.Six components gentiopicroside,ligustrazine,sesamin,osthol,asarone,and columbianadin were identified.The similarity between the fingerprints of Duhuo Jisheng Pills of 15 batches and the control fingerprints were all above 0.900.Duhuo Jisheng Pills of 15 batches could be divided into two categories,S1,S4,and S6 as one category,while the other samples belong to another category.Nine principal component factors can be used as evaluation indicators for Duhuo Jisheng Pills.The three variables that contribute significantly to model classification were the common peaks 4,17,and 2.Conclusion The method can be used to evaluate the quality of Duhuo Jisheng Pills,which can provide a basis for the study of quality standards for Duhuo Jisheng Pills.

关 键 词：独活寄生丸 指纹图谱 龙胆苦苷 二氢欧山芹醇当归酸酯 聚类分析 主成分分析 正交偏最小二乘判别分析 

分 类 号：R286.02[医药卫生—中药学]