High yield synthesis of nigerooligosaccharides by transglycosylation catalyzed by α-glucosidase TaAglA from Thermoplasma acidophilum  

作　　者：Wei Xia Yifan Wang Ran Bi Jing Wu 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,214122,China [2]School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education,Jiangnan University,1800 Lihu Avenue,Wuxi,214122,China [3]International Joint Laboratory on Food Safety,Jiangnan University,1800 Lihu Avenue,Wuxi,214122,China

出　　处：《Food Bioscience》2022年第3期245-252,共8页食品生物科学（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(31730067,31801472);the Natural Science Foundation of Jiangsu Province(BK20180604);the national first-class discipline program of Light Industry Technology and Engineering(LITE2018-03).

摘　　要：Nigerooligosaccharides (NOS) is a new functional oligosaccharide containing α-1,3 glucosidic bond with good anti-digestive properties and intestinal probiotics.Transglycosylation catalyzed by α-glucosidase is an effective method for the preparation of oligosaccharides.However,there are few reports on the enzymatic synthesis of nigerooligosaccharides by α-glucosidase at present.This study was aimed to investigate the transglycosylation property of the GH31 α-glucosidase from Thermoplasma acidophilum,TaAglA,and also evaluate its application performance in the preparation of NOS.It was found that TaAglA exhibited selectivity for α-1,3 and α-1,4 linkages when catalyzing hydrolysis,but for α-1,3 and α-1,6 linkages when catalyzing transglycosylation.Using 10% glucose and 20% maltose as substrates,TaAglA yielded 88.5 g/L NOS under the condition of pH 6.0,80 ℃ and 1 U/mL enzyme addition,which was the highest level to our knowledge.In addition,components with higher polymerization degrees,i.e.nigerotriose and nigerosyl-glucose,occupied 53.6% proportion of the total NOS products,giving it better probiotic functions.Futhermore,after purification by glucoamylase digestion and yeast culture,the final yield of NOS was 26.1%,and the purity of the product was 93%.These findings on TaAglA were expected to provide a new candidate for large-scale enzymatic synthesis of NOS,and also have important theoretical significance for the study of GH31 α-glucosidase.

关 键 词：Nigerooligosaccharides Α-GLUCOSIDASE α-1 3 glucosidic bond Enzymatic synthesis TRANSGLYCOSYLATION 

分 类 号：O62[理学—有机化学]