我国沈阳一起GI.6型札如病毒引起的胃肠炎暴发的分子病原学特征研究  

Molecular etiological study on the GI.6 Norovirus causing an infectious diarrhea outbreak in Shenyang,China

作　　者：田军[1] 丛实 陈玲 梁佳元[3] 王冰[4] Tian Jun;Cong Shi;Chen Ling;Liang Jiayuan;Wang Bing(Third People′s Hospital of Dalian,Dalian 116000,China;Shengyang Medical College,Shenyang 110034,China;Liaoning Provincial Certer for Disease Control and Prevention,Shenyang 110000,China;Shenyang Center for Disease Control and Prevention,Shenyang 110000,China)

机构地区：[1]大连市第三人民医院,大连116000 [2]沈阳医学院,沈阳110034 [3]辽宁省疾病预防控制中心,沈阳110000 [4]沈阳市疾病预防控制中心,沈阳110000

出　　处：《中华实验和临床病毒学杂志》2023年第3期310-314,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科技重大专项课题(2017ZX10103007-001);沈阳市科技攻关计划项目(21-173-9-03)。

摘　　要：目的明确我国沈阳一起急性胃肠炎暴发的病原学及其基因进化特征。方法采集2021年11月沈阳某高校暴发疫情中的学生病例肛拭子和环境涂抹标本共计57份,采用实时荧光RT-PCR进行常规腹泻病毒基因检测,并对札如病毒核酸阳性标本采用传统RT-PCR方法扩增、序列测定、拼接和遗传进化分析。结果实时荧光RT-PCR检测确定26份标本为札如病毒核酸阳性,成功扩增15份标本的衣壳蛋白基因片段。遗传进化分析表明,15个阳性标本札如病毒分子分型均为GI.6基因型,各标本序列间同源性为99.99%,与2005日本的LC380411毒株亲缘较近为98.84%。结论引起本次暴发的病原札如病毒在东北地区较少检出,其基因型为GI.6亚型引起暴发全球罕见报道。Objective To identify the molecular pathogenic characteristics and genetic evolution of an outbreak of acute gastroenteritis in Shenyang,China.Methods A total of 57 anal swabs and environmental swab samples from student cases in a university outbreak in Shenyang in November 2021 were collected.Fluorescence quantitative RT-PCR was used for routine detection of diarrheal virus genes,and traditional RT-PCR was used to amplify and sequence the positive specimens of Norovirus.Phylogenetic analysis was performed.Results Of the 57 specimens,26 were norovirus positive by fluorescence quantitative real-time PCR,and 15 specimens were successfully amplified for the capsid protein gene segment.Phylogenetic analysis showed that all 15 positive specimens were of the GI.6 genotype of Norovirus,with a homology of 99.99%among the specimens and a genetic similarity of 98.84%to the LC380411 strain in Japan in 2005.Conclusions The pathogen of this outbreak,GI.6 Norovirus,is rarely detected in the northeast of China,and its genotype is a subtype that has rarely been reported worldwide.

关 键 词：札如病毒 胃肠炎暴发 GI.6型 遗传进化 

分 类 号：R512.5[医药卫生—内科学] R446.5[医药卫生—临床医学]