中国人乳头瘤病毒30型分布及型下分类研究  

Study on the distribution and lineages classification of human papillomavirus type 30 in China

作　　者：李颖[1] 郭一帆 王慧[1] 王娇[1] 张杰琼 刘宏图[1] Li Ying;Guo Yifan;Wang Hui;Wang Jiao;Zhang Jieqiong;Liu Hongtu(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of Obstetrics and Gynaecology,Hainan Hospital of Chinese PLA General Hospital,Sanya 572013,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所、国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]中国人民解放军总医院海南分院妇产科,三亚572013

出　　处：《中华实验和临床病毒学杂志》2023年第3期315-319,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解国内人乳头瘤病毒(human papillomavirus,HPV)30型(HPV30)的感染情况及型下系(lineage)分类。方法收集就诊于中国人民解放军总医院妇产科,并在30d内进行新柏氏薄层液基细胞学检测女性患者宫颈脱落细胞样品,从中提取DNA并进行HPV30型特异性聚合酶链式反应(polymerasechainreaction,PCR)检测;对HPV30阳性样本进行病毒基因组全长PCR扩增和序列分析。结果1600例受试者中,共获得HPV30阳性样品9例(0.56%,9/1600),其中6例HPV30基因组全长PCR扩增成功;9例HPV30毒株在型下系分类上都属于A,其中A1占66.7%(6/9)、A4占22.2%(2/9)、A5占11.1%(1/9)。结论国内女性中存在HPV30感染,型下系分类上全部HPV30毒株都属于A。Objective To investigate the infection rate and intra-type lineage distribution of human papillomavirus type 30(HPV30)in Chinese women.Methods Cervical exfoliated cells were collected within 30 days of cytological tests for patients from the People’s Liberation Army General Hospital.After DNA extraction and screening of HPV30 type specific polymerase chain reaction(PCR),full-length genomes were amplified for HPV30 positive samples.Results A total of 1600 cervical samples were collected.The infection rate of HPV30 was 0.56%(9/1600).All the nine HPV30 strains belonged to lineage A.A1 accounted for 66.7%(6/9),A4 accounted for 22.2%(2/9),A5 accounted for 11.1%(1/9).Conclusion HPV30 infection exists among Chinese women.Lineage A was the only prevalent lineage across all the HPV30 isolates.

关 键 词：宫颈癌 人乳头瘤病毒30型 分类 

分 类 号：R711.32[医药卫生—妇产科学]