黄芪甲苷对人源宫颈癌细胞C4-1的生长抑制作用及机制研究  

Investigation on the inhibitory effect of astragaloside IV on the growth of cervical cancer cell C4-1 and the action mechanism

作　　者：牟坤 杨福利 郭晓青 谷翠华 亓久德 MOU Kun;YANG Fuli;GUO Xiaoqing;GU Cuihua;QI Jiude(Department of Oncology,the Affiliated People’s Hospital of Shandong First Medical University,Jinan 271199,China)

机构地区：[1]山东第一医科大学附属人民医院肿瘤科,山东济南271199

出　　处：《中国优生与遗传杂志》2023年第6期1109-1113,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究黄芪甲苷对人源宫颈癌细胞C4-1接种的裸鼠移植瘤的生长抑制作用并探讨其作用机制。方法Balb/C裸鼠随机分为对照组(不做建模处理)、模型组和黄芪甲苷低剂量组、黄芪甲苷高剂量组,C4-1皮下接种裸鼠建立宫颈癌模型,黄芪甲苷干预治疗后,检测各组小鼠的体质量、肿瘤体积、抑瘤率、血清中MMP-9、Gal-3和VEGF水平、肿瘤组织中微血管密度、miR-21和Stat-3基因表达,以及肿瘤组织中VEGFR和Stat-3蛋白表达水平。结果与对照组相比,模型组小鼠血清中MMP-9、Gal-3和VEGF水平、组织中微血管密度、肿瘤组织中miR-21和Stat-3基因表达水平、VEGFR和Stat-3蛋白表达水平均显著升高(P<0.05);与模型组相比,黄芪甲苷干预治疗后肿瘤体积、MMP-9、Gal-3和VEGF水平、组织中微血管密度、肿瘤组织中miR-21和Stat-3基因表达水平,以及肿瘤组织中VEGFR和Stat-3蛋白均显著减小(P<0.05),高剂量组上述作用效果均显著高于低剂量组(P<0.05),且高剂量组抑瘤率显著大于低剂量组(P<0.05)。结论黄芪甲苷对人源宫颈癌细胞C4-1具有显著的抑制作用,其作用机制可能为通过降低肿瘤组织中miR-21和Stat-3基因表达而抑制肿瘤组织中血管新生。Objective To investigate the inhibitory effect of astragaloside on the growth of C4-1(human-derived cervical cancer cells lined)cell-transplanted tumors in the nude mice,and further to discuss the action mechanism.Methods Balb/C nude mice were randomly divided into control group(no modeling treatment),model group and astragaloside low and high dose groups.C4-1 subcutaneously inoculated nude mice were used to establish cervical cancer model,and after astragaloside intervention treatment,the body weight,tumor volume,tumor inhibition rate,as well as MMP-9,Gal-3 and VEGF levels in serum,microvessel density in tumor tissue,miR-21 and Stat-3 genes expression,VEGFR and Stat-3 protein expression in tumor tissues were detected in each group.Results Compared with the control group,MMP-9,Gal-3 and VEGF levels in serum,microvessel density in tissues,and the expression of miR-21 and Stat-3,and the expression of VEGFR and Stat-3 proteins were significantly increased in model group(P<0.05).Compared with the model group,the tumor volume,the MMP-9,Gal-3 and VEGF levels in serum,microvessel density in tissue,miR-21 and Stat-3 gene expression in tumor tissue and the expression level of VEGFR and Stat-3 protein in tumor tissue were significantly reduced after astragaloside treatment(P<0.05),and the above effects were significantly higher in the high-dose group than in the low-dose group(P<0.05),and the tumor suppression rate in the high-dose group was significantly higher than that in the low-dose group(P<0.05).Conclusion Astragaloside IV exhibits significant inhibitory effects on the growth of cervical cancer cell C4-1,and the mechanism may be related to inhibit angiogenesis in tumor tissue by reducing the expression of miR-21 and Stat-3 genes.

关 键 词：黄芪甲苷 人源宫颈癌细胞C4-1 肿瘤生长抑制 miR-21和Stat-3基因 作用机制 

分 类 号：R285[医药卫生—中药学]