聚己内酯/钛酸钡纳米纤维膜的制备及其力学性能和细胞活性  

Preparation,mechanical properties and cytoactive of polycaprolactone/BaTiO_(3) nanofiber membranes

作　　者：瞿洋洋 覃再嫩 朱必康 罗世兴[1,3] 梁锐明 Qu Yangyang;Qin Zainen;Zhu Bikang;Luo Shixing;Liang Ruiming(Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry,Guangxi Medical University,Nanning 530021,China;Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration,Guangxi Medical University,Nanning 530021,China;Beihai People's Hospital,Beihai 536000,China)

机构地区：[1]广西医科大学再生医学与医用生物资源开发应用省部共建协同创新中心,南宁530021 [2]广西医科大学广西组织器官修复医用生物材料工程技术研究中心,南宁530021 [3]北海市人民医院,北海536000

出　　处：《广西医科大学学报》2023年第4期580-585,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金地区基金资助项目(No.81960414);广西自然科学基金资助项目(No.2020GXNSFBA238011)。

摘　　要：目的:研究聚己内酯/钛酸钡[PCL/BaTiO3(PCL/BT)]纳米纤维膜的制备及其力学性能和细胞活性。方法:通过加入BaTiO3,利用静电纺丝技术制成PCL、PCL/3%BT、PCL/6%BT和PCL/9%BT纳米纤维膜。扫描电镜(SEM)观察膜片形貌及间充质干细胞的生长形态,力学性能实验检测材料的机械性能。CCK-8法检测间充质干细胞在膜上7 d间充质干细胞的增殖情况。SEM下观察细胞活力,探究骨髓间充质干细胞在电纺纳米纤维膜上的生长情况。Calcein-AM/PI染色比较间充质干细胞与纳米纤维膜复合培养7 d时细胞存活情况。结果:SEM观察结果显示,纯PCL静电纺丝膜纤维表面光滑,未发现凹陷或凸出,PPCL/3%BT静电纺丝膜纤维表面偶有突出,PCL/6%BT静电纺丝膜纤维变得稀疏,PCL/9%BT静电纺丝膜纤维表面有明显的沉淀物,并且明显可以看出PCL/6%BT的孔隙率优于其他组。间充质干细胞在PCL/6%BT膜上的细胞增殖和活性显著优于其他3种膜片,细胞分布更密集。结论:本研究成功地利用静电纺丝技术制备出PCL、PCL/3%BT、PCL/6%BT和PCL/9%BT纳米纤维膜,该纳米纤维膜具有良好机械性能,并具有促进细胞增殖能力。Objective:To study the preparation,mechanical properties and cellular activities of polycaprolactone/BaTiO3[PCL/BaTiO3(PCL/BT)]nanofiber membranes.Methods:PCL,PCL/3%BT,PCL/6%BT and PCL/9%BT nanofiber membranes were made by adding BaTiO3 using electrospinning technology.Scanning electron microscopy(SEM)observed the diaphragm morphology and the growth morphology of bone marrow mesenchymal stem cells(MSCs),and mechani-cal properties tested the mechanical properties of materials.MSC proliferation of bone marrow on the membrane at 7 days was measured by CCK-8 assay.Cell viability was observed under SEM to explore the growth of bone marrow mesenchymal stem cells(BMMSCs)on electrospun nanofiber membranes.Calcein-AM/PI staining was used to compare cell survival of bone marrow MSCs in compound culture with nanofiber membranes for 7 d.Results:SEM results showed that the surface of pure PCL electrospinning film fiber was smooth with no depression or protrusion,PCL/3%BT electrospinning film fiber surface had protrusion occasionally,PCL/6%BT electrospinning film fiber became sparse,and PCL/9%BT electrospinning film fiber surface was precipitated obviously.It was obvious that the porosity of PCL/6%BT was better than that of other groups.Bone marrow MSCs had significantly better cell proliferation and activity on PCL/6%BT membranes than the other three membranes and a more dense cell distribution.Conclusion:In this study,PCL,PCL/3%BT,PCL/6%BT and PCL/9%BT nanofiber membranes are successfully prepared using electrospinning technology.The nanofiber membranes have good mechanical properties and have the ability to promote cell proliferation.

关 键 词：聚己内酯 钛酸钡纳米纤维膜 静电纺丝 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]