LRG1在脓毒症急性肺损伤大鼠中的表达  

Study on the expression of LRG1 in rats with acute lung injury caused by sepsis

作　　者：李宛霓 龙启成[2] 廖品琥[1] Li Wanni;Long Qicheng;Liao Pinhu(Department of Emergency Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Department of Critical Care Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院急诊科,南宁530021 [2]广西医科大学第一附属医院重症医学科,南宁530021

出　　处：《广西医科大学学报》2023年第4期651-656,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.82060022)。

摘　　要：目的:探讨富含亮氨酸α-2糖蛋白(LRG1)在脓毒症急性肺损伤大鼠中的表达。方法:24只SPF级雄性SD大鼠随机分为对照组、脓毒症肺损伤6 h组(6 h组)、脓毒症肺损伤12 h组(12 h组)、脓毒症肺损伤24 h组(24 h组),每组6只大鼠,用腹腔注射脂多糖(LPS,10 mg/kg)的方法建立脓毒症急性肺损伤大鼠模型,对照组腹腔注射等剂量生理盐水(10 mL/kg)。实时荧光定量PCR(RT-qPCR)检测肺组织中LRG1 mRNA的表达,酶联免疫吸附试验(ELISA)测定大鼠血清中LRG1浓度,苏木精—伊红(HE)染色观察肺组织病理特征,免疫组织化学染色观察LRG1在肺组织中的定位表达,western blotting检测肺组织中LRG1蛋白的表达。结果:与对照组相比,脓毒症急性肺损伤大鼠肺组织出现炎症细胞浸润,肺泡壁增厚,肺泡间隔增宽,符合脓毒症肺损伤的特点。LRG1在肺泡上皮细胞和炎症浸润的免疫细胞中均有表达。与对照组相比,6 h组、12 h组、24 h组大鼠血清中的LRG1水平升高(P<0.05);6 h组大鼠肺组织中LRG1 mRNA相对表达量高于对照组(P<0.05);24 h组大鼠肺组织中LRG1蛋白表达高于对照组(P<0.05)。结论:LRG1在脓毒症急性肺损伤大鼠模型中的表达水平升高。Objective:To explore the expression of Leucine-rich alpha-2-glycoprotein 1(LRG1)in rats with acute lung injury caused by sepsis.Methods:24 SPF male SD rats were randomly divided into control group,6 h group with sepsis lung injury(6 h group),12 h group with sepsis lung injury(12 h group)and 24 h group with sepsis lung injury(24 h group),with 6 rats in each group.The rat model of acute lung injury with sepsis was established by intraperitoneal injection of lipopolysaccharide(LPS,10 mg/kg).The rats in the control group were intraperitoneally injected with the same dose of normal saline(10 mL/kg).The expression of LRG1 mRNA in lung tissue was detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).The concentration of LRG1 in rat serum was determined by enzyme-linked immunosorbent assay(ELISA).The pathological characteristics of lung tissue were observed by hematoxylin-eosin(HE)staining.The protein expression of LRG1 in lung tissue was detected by immunohistochemical staining.The expression of LRG1 protein in lung tissue was detected by western blotting.Results:Compared with the control group,the lung tissue of the rats with acute lung injury caused by sepsis showed inflammatory cell infiltration,thickening of the alveolar wall,and widening of the alveolar septum,which was consistent with the characteristics of lung injury caused by sepsis.LRG1 was expressed in both alveolar epithelial cells and inflammation-infiltrated immune cells.Compared with the control group,the serum of the rats of LRG1 levels of the 6 h group,12 h group and the 24 h group increased(P<0.05);the relative expression level of LRG1 mRNA in the lung tissue of the rats of the 6 h group was higher than that of the control group(P<0.05);LRG1 protein expression level in the lung tissue of the rats of the 24 h group was higher than that of the control group(P<0.05).Conclusion:LRG1 expression increases in the rat model of acute lung injury caused by sepsis.

关 键 词：脓毒症 急性肺损伤 富含亮氨酸α-2糖蛋白 炎症反应 

分 类 号：R563.8[医药卫生—呼吸系统]