木薯MeSAUR1互作蛋白的筛选及鉴定  

Screening and Identification of MeSAUR1 Interacting Protein in Cassava

作　　者：郑婉茹 丁凯旋 陆小花 王睿[1,2] 陈银华[1,2] 姚远[3] 陈新[3] 耿梦婷[1,2] Zheng Wanru;Ding Kaixuan;Lu Xiaohua;Wang Rui;Chen Yinhua;Yao Yuan;Chen Xin;Geng Mengting(College of Tropical Crops,Hainan University,Haikou,570228;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources,College of Tropical Crops,Hainan University,Haikou,570228;Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Hainan Seed Station,Haikou,571100)

机构地区：[1]海南大学热带作物学院,海口570228 [2]海南大学热带作物学院,海南省热带生物资源可持续利用国家重点实验室培育基地,海口570228 [3]中国热带农业科学院热带生物技术研究所,海口571101 [4]海南省种子总站,海口571100

出　　处：《分子植物育种》2023年第14期4659-4665,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(No.31960058);海南省研究生创新科研课题(No.Hys2020-140)共同资助。

摘　　要：木薯MeAGPS1a基因编码的小亚基是淀粉合成关键酶AGPase的催化中心,前期研究发现MeSAUR1转录因子可结合MeAGPS1a基因启动子并调控该基因表达。采用酵母双杂交技术筛选MeSAUR1转录因子的互作蛋白,可进一步解析MeAGPS1a基因表达的分子调控网络。本研究构建了MeSAUR1的酵母双杂交诱饵载体pGBKT7-MeSAUR1,采用酵母双杂交技术从木薯cDNA文库中筛选MeSAUR1的候选互作蛋白,结果共筛选出31个阳性克隆。通过酵母双杂交点对点实验验证了候选互作蛋白MePP2C与MeSAUR1的互作关系,本研究结果有助于揭示Me SAUR1蛋白调控木薯淀粉合成的互作网络。The small subunit enco ded by MeAGPS1a gene is the catalytic center of AGPase in cassava,and AGPase is a key enzyme in starch synthesis.Previous studies have found that MeSAUR1 transcription factor can bind to the promoter of MeAGPS1a gene and regulate its expression.Screening the interacting protein of MeSAUR1 transcription factor by yeast two-hybrid technology can further analyze the molecular regulatory network of MeAGPS1a gene expression.The yeast two-hybrid vector pGBKT7-MeSAUR1 of MeSAUR1 was constructed in this study,and the candidate interacting proteins of MeSAUR1 were screened from cassava cDNA library by yeast two-hybrid technology,and 31 positive clones were screened.The interaction between candidate interacting protein MePP2C and MeSAUR1 was verified by yeast two-hybrid point-to-point experiment.The results of this study are beneficial to reveal the interacting network of MeSAUR1 protein regulating starch synthesis in cassava.

关 键 词：木薯 MeSAUR1 酵母双杂交 互作蛋白 

分 类 号：S533[农业科学—作物学]