基于高密度遗传连锁图谱的稻谷粒重QTL定位  

QTLs Mapping of Rice Grain Weight Based on High-density Genetic Map

作　　者：张大双 彭强 李佳丽 季家举 徐海峰 朱速松[1] Zhang Dashuang;Peng Qiang;Li Jiali;Ji Jiaju;Xu Haifeng;Zhu Susong(Guizhou Rice Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang,550006;Hunan Light Industry Institute,Changsha,410015)

机构地区：[1]贵州省农业科学院贵州省水稻研究所,贵阳550006 [2]湖南轻工研究院有限责任公司,长沙410015

出　　处：《分子植物育种》2023年第14期4679-4684,共6页Molecular Plant Breeding

基　　金：贵州省省级科技计划项目(黔科合基础-ZK[2023]一般178);国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401);黔水稻所青年基金项目([2021]005号)共同资助。

摘　　要：为挖掘稳定遗传的稻谷粒重性状QTL,本研究以V20B/CPSLO17组合衍生的150个重组自交家系(recombinant inbred line,RIL)为作图群体,在3个环境(2019贵阳,2020贵阳,2019三亚)对稻谷粒重性状进行QTL检测及其遗传效应分析。结果表明:3个环境共检测到6个稻谷粒重QTL,其中QTL qTGW5-1在2种环境被重复检测到;QTL qTGW5-2和qTGW5-3具有较大遗传效应,表型变异贡献率高达139.796%和99.414%,两者的LOD值分别为35.113和28.411。qTGW5-2的加性效应源自亲本CPSLO17;qTGW5-3的加性效应源自亲本V20B。本研究结果为挖掘新的稻谷粒重性状基因提供参考依据。In order to explore the stable genetic QTLs for grain weight traits of rice,150 recombination inbred lines(RILs)derived from V20B/CPSLO17 combination,were applied to detect QTLs for grain weight traits and analyze their genetic effects in three environments(2019 Guiyang,2020 Guiyang,2019 Sanya).The results showed that a total of six QTLs for grain weight traits were detested in three environments,of which the QTL qTGW5-1 was repeatedly detected in two environments.QTLs qTGW5-2 and qTGW5-3 had major genetic effects,and the contribution rate of phenotypic variation was as high as 139.796% and 99.414%,and the LOD values of the two were 35.113 and 28.411,respectively.The additive effect of qTGW5-2 was derived from the parent CPSLO17,and qTGW5-3 was derived from the parent V20B.These results provide reference basis for further exploiting new candidate gene of grain weight in rice.

关 键 词：水稻(Oryza sativa L.) 环境 粒重 作图群体 基因 

分 类 号：S511[农业科学—作物学]