黄淮麦区(南片)小麦穗发芽抗性评价及其等位基因检测  

Evaluation of Wheat Pre-harwest Sprouting Resistance and Allele Detection in Huanghuai Southern Wheat Region

作　　者：陈杰 张星宇 白冬 宋佳静 宋全昊 赵立尚 朱统泉 朱保磊 陈建辉 张香粉 Chen Jie;Zhang Xingyu;Bai Dong;Song Jiajing;Song Quanhao;Zhao Lishang;Zhu Tongquan;Zhu Baolei;Chen Jianhui;Zhang Xiangfen(Zhumadian Agricultural Academy of Sciences,Zhumadian,463000;College of Agronomy,Henan Agricultural University,Zhengzhou,450002;Xinyang Agricultural Academy of Sciences,Xinyang,464000;Henan Science and Technology Exchange Center with Foreign Countries,Zhengzhou,450003;Henan Seed Station,Zhengzhou,450003)

机构地区：[1]河南省驻马店市农业科学院,驻马店463000 [2]河南农业大学农学院,郑州450002 [3]河南省信阳市农业科学院,信阳464000 [4]河南省对外科技交流中心,郑州450003 [5]河南省种子管理站,郑州450003

出　　处：《分子植物育种》2023年第14期4694-4701,共8页Molecular Plant Breeding

基　　金：国家现代农业产业技术体系项目(CARS-03);河南省小麦重大科技专项(151100110400);驻马店市科技攻关项目(14103,15124,16108,17137)共同资助。

摘　　要：为了解黄淮麦区(南片)当前小麦穗发芽抗性以及等位基因的分布情况,本研究以94份黄淮麦区(南片)小麦新品系为供试材料,利用种子发芽指数法对供试材料的穗发芽抗性进行评价,同时利用功能标记Vp1B3和Dorm-1对供试材料Vp1B和Dorm-B1位点等位基因进行检测。结果表明,供试材料发芽指数(GI)总体平均值为39.6%,标准差为18.08,变幅为9.62%~88.48%,这批供试材料的穗发芽抗性以中感型为主;在Vp1B位点,共检测到Vp1Ba和Vp1Bc两种等位基因,分布频率分别为64.89%和35.11%,携带等位基因Vp1Ba材料的发芽指数(GI)显著高于Vp1Bc等位基因(P<0.05),等位基因Vp1Bc与抗穗发芽相关,等位基因VP-1Ba与感穗发芽相关;在Dorm-B1位点,仅检测到Dorm-B1a等位基因,这批供试材料Dorm-B1位点的多态性较为单一。本研究能够为黄淮麦区(南片)小麦穗发芽分子改良提供参考信息。In order to understand the distribution of the pre-harwest sprouting genes in new bread wheat cultivars from Huanghuai southern region.In this study,a total of 94 wheat varieties newly cultivated in the Huanghuai southern region were used as the test materials.The seed germination index method was used to evaluate the resistance of the tested materials to the pre-harwest sprouting,and the functional markers Vp1B3 and Dorm-1 were used to detect the alleles of Vp1B and Dorm-B1.The results showed that the average value of germination index(GI)was 39.6%,the standard deviation was 18.08,and the range was 9.62%~88.48%;In the Vp1B locus,the frequency of the tested wheat varieties with Vp1Ba allele was 64.89%,and Vp1Bc allele was 35.11%.The germination index(GI)of the Vp1Ba is significantly higher than that of Vp1Bc(P<0.05),Vp1Bc allele was associated with resistant sprouting,and Vp1Ba allele was associated with susceptible sprouting;In the Dorm-B1 locus,only Dorm-B1a alleles were detected,and the polymorphism of Dorm-B1 locus in these materials was relatively single.This study can provide reference information for the molecular improvement of the pre-harwest sprouting in Huanghuai southern region.

关 键 词：小麦 穗发芽 发芽指数 功能标记 

分 类 号：S512.1[农业科学—作物学]