不同种群罗氏沼虾幼体的肠道菌群结构与功能特征分析  

Structural and Functional Characteristics of Intestinal Microbiota in Different Populations of Macrobrachium rosenbergii Larvae

作　　者：施金谷 武霞 黄光华 杨慧赞 梁怡 吕敏 曾兰 胡大胜 黄立斌 王瑞 SHI Jingu;WU Xia;HUANG Guanghua;YANG Huizan;LIANG Yi;LYU Min;ZENG Lan;HU Dasheng;HUANG Libin;WANG Rui(Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture,Guangxi Academy of Fishery Sciences,Nanning 530021,China;Fisheries Technology Extension Center of Guangxi,Nanning 530022,China;College of Life Sciences,Guangxi Normal University,Guilin 541006,China)

机构地区：[1]广西水产科学研究院,广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]广西水产技术推广站,广西南宁530022 [3]广西师范大学生命科学学院,广西桂林541006

出　　处：《水产科学》2023年第4期674-681,共8页Fisheries Science

基　　金：广西重点研发项目(桂科AB1850016,桂科AB19245033);广西科技重大专项(桂科AA17204095-5)。

摘　　要：相同养殖条件下,养殖5个种群的罗氏沼虾,选取同批次孵化20 d的罗氏沼虾幼体,采用16S rRNA高通量测序技术对罗氏沼虾缅甸种群、斯里兰卡种群、越南种群、孟加拉种群和自有品种1种群幼虾的肠道菌群进行分析。结果显示,罗氏沼虾缅甸种群、斯里兰卡种群、越南种群、孟加拉种群和自有品种1种群幼虾5个组共有的运算分类单元为256个,每组特有的运算分类单元分别为131、247、231、144、184个。孟加拉种群幼虾的肠道菌群物种丰富度最高,越南种群幼虾的肠道菌群物种多样性最高。5个组的优势菌群在门、科水平上都相似,都为变形菌门、拟杆菌门和厚壁菌门,但其丰度及其他菌群的组成存在差异,斯里兰卡种群肠道菌群中厚壁菌门的丰度大于拟杆菌门,与其他4组菌群不同。5个组的菌群功能也存在共性,主要集中于新陈代谢中的碳水化合物代谢、氨基酸代谢、维生素代谢等方面。试验结果表明,罗氏沼虾不同种群幼体的肠道菌群在结构组成、丰度、代谢功能等方面存在一定的共性。本研究结果可为进一步研究不同种群罗氏沼虾的生长发育机制打下基础。Under the same culture conditions,five populations of Macrobrachium rosenbergii were cultured.The larvae of M.rosenbergii hatched for 20 days in the same batch were selected.The intestinal microflora of the larvae of Burmese population,Sri Lankan population,Vietnamese population,Bengali population and own variety 1 population were analyzed by 16S rRNA high-throughput sequencing technology.The results showed that there were 256 OTUs in common in five groups,and the unique OTUs of each group were 131,247,231,144 and 184,respectively.The species richness of intestinal microbiota in Bengali population was the highest,and the species diversity of gut microbiota in Vietnamese population was the highest.The dominant microflora of five groups were similar in phylum and family level,which were Proteobacteria,Bacteroidetes and Firmicutes,but their abundance and composition of other microflora were different.The abundance of Firmicutes in intestinal microflora of Sri Lankan population was higher than that of Bacteroidetes,which was different from the other four groups.The five groups also have common functions,mainly focusing on carbohydrate metabolism,amino acid metabolism,vitamin metabolism and so on.The results showed that the intestinal microbiota of different populations of M.rosenbergii had some similarities in structure,abundance and metabolic function.This study analyzed the structure and functional characteristics of intestinal microbiota of different populations of M.rosenbergii larvae,which laid a foundation for further study on the growth and development mechanism of different populations of M.rosenbergii.

关 键 词：16S rRNA 罗氏沼虾 肠道菌群 菌群功能 

分 类 号：S917.1[农业科学—水产科学]