仿刺参水管系统与体腔间物质交换问题探究  

Substance Exchange between Water-Vascular System and Coelom in Sea Cucumber Apostichopus japonicus

作　　者：范栩源 王振辉 任媛 国丽媛 孙启睿 王轶南 李强 FAN Xuyuan;WANG Zhenhui;REN Yuan;GUO Liyuan;SUN Qirui;WANG Yinan;LI Qiang(Dalian Ocean University,Dalian 116023,China;College of Marine and Biology Engineering,Yancheng Institute of Technology,Yancheng 224051,China;School of Bioengineering,Dalian University of Technology,Dalian 116024,China)

机构地区：[1]大连海洋大学,辽宁大连116023 [2]盐城工学院海洋与生物工程学院,江苏盐城224051 [3]大连理工大学生物工程学院,辽宁大连116024

出　　处：《水产科学》2023年第4期705-711,共7页Fisheries Science

基　　金：国家自然科学基金资助项目(42076112,31872544)。

摘　　要：仿刺参具有复杂的水管系统,其内充满体腔液和体腔细胞,但与体腔中的体腔液及体腔细胞存在显著差异。为进一步探究仿刺参水管系统与体腔的关联性及连通性,利用多种物质进行体腔注射,通过检测注射物在体腔和水管系统(以波里氏囊为代表)内的分布,分析仿刺参水管系统与体腔间物质交换问题。试验结果显示:注射甜菜红后6 h,体腔液和波里氏囊腔液(简称囊腔液)均被着色;注射谷胱甘肽硫基转移酶标签蛋白后6 h和12 h,体腔液中可检测到大量外源蛋白,囊腔液中也有外源蛋白存在;注射荧光微球后6 h和12 h,体腔液中可观察到大量荧光微球分布,而囊腔液中未发现;注射大肠杆菌和灿烂弧菌后6、12、24、72 h,体腔液中均有大量细菌分布,而囊腔液中未分离到细菌。试验结果表明,仿刺参水管系统和体腔间并非无限畅通,小分子和生物大分子等可溶性物质可以流通于体腔和水管系统之间,但外源颗粒物和细菌则不能通过体腔进入水管系统。试验结果可为后续研究仿刺参水管系统的生理结构和在机体中的生物学作用提供基础数据。Sea cucumber Apostichopus japonicus with a body mass of(65±10)g was injected in coelom with beet root pigments-beetroot red,purified and adjusted GST-tagged protein,fluorescent microsphere dilution,suspension of Vibrio splendidus and suspension of Escherichia coli.The distribution of the injected matters in the coelom and the water-vascular system(represented by polian vesicle)was detected to explore the correlation,connectivity and material exchange between the coelom and the water-vascular system of the sea cucumber.The results showed that the coelomic fluid from coelom and polian vesicle(hereinafter referred to as“vesicle fluid”)were stained into red at 6 hours after injection by beet red dye.A large number of GST-tag proteins were detected in the coelomic fluid,and there were also some proteins in the vesicle fluid 6 h and 12 h after protein injection.Many fluorescent microspheres were observed in the coelomic fluid,but not in the vesicle fluid 6 h and 12 h after injection.In addition,a large number of E.coli and V.splendidus bacteria were found in the coelomic fluid 6,12,24 and 72 h after injection,without bacteria isolated in the vesicle fluid.In conclusion,the water-vascular system and the coelom are not found to be infinitely unblocked in sea cucumber.Soluble substances such as small molecules and biological macromolecules were exchanged between the coelom and the water-vascular system,but the exogenous particles and bacteria did not enter the water-vascular system via the coelom.The findings laid a theoretical foundation for the subsequent study of the physiological structure and biological role of the water-vascular system in sea cucumber.

关 键 词：仿刺参 水管系统 波里氏囊 体腔 物质交换 

分 类 号：S968.9[农业科学—水产养殖]