MMP15、PICK1基因在山羊睾丸中的表达研究  

Expression of MMP15 and PICK1 Genes in Goat Testis

作　　者：汪艳妃 张艳[1] 惠茂茂 陈祥[1] Wang Yanfei;Zhang Yan;Hui Maomao;Chen Xiang(Key Laboratory of Plateau Mountain Animal Genetics,Breeding and Reproduction,Ministry of Education/Guizhou Provincial Key Laboratory of Animal Genetics,Breeding and Reproduction/College of Animal Science,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室/动物科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2023年第4期18-25,共8页Journal of Mountain Agriculture and Biology

基　　金：国家重点研发计划项目(2021YFD1200403);国家自然科学基金项目(32060753);贵州省科技支撑计划项目(黔科合支撑[2021]一般151)。

摘　　要：MMP15、PICK1基因均是哺乳动物性腺组织中影响性腺生理功能正常发挥的重要影响因子,但它们在黔北麻羊睾丸组织中的表达规律尚不明晰。为研究黔北麻羊不同月龄的睾丸中MMP15、PICK1基因的表达情况,本试验以黔北麻羊为研究对象,通过qRT-PCR技术分别检测MMP15与PICK1基因在1、3、6、9、12月龄的黔北麻羊睾丸中的表达水平并建立基因表达的标准曲线。结果显示:MMP15、PICK1基因在黔北麻羊不同月龄睾丸组织中均有表达;MMP15基因在黔北麻羊不同月龄睾丸中的表达量从高到低排列为:12月龄>1月龄>9月龄>3月龄>6月龄;12月龄与1月龄的表达差异不显著(P>0.05),二者表达量均极显著高于3月龄、6月龄、9月龄(P<0.01);9月龄表达显著高于3月龄(P<0.05),极显著高于6月龄(P<0.01);3月龄表达极显著高于6月龄(P<0.01)。PICK1基因在黔北麻羊不同月龄睾丸中的表达量从高到低排列分别是:3月龄>6月龄>12月龄>9月龄>1月龄;3、6月龄其表达量极显著高于9、12月龄(P<0.01);1月龄与其他几个月龄相比,其表达量均极显著低于其他月龄(P<0.01)。结合黔北麻羊的生理机能发育情况,黔北麻羊在6月龄时已经基本达到性成熟,所以此时睾丸内细胞活动增强,睾丸机能活跃。本次试验结果表明,黔北麻羊睾丸生精功能的正常发挥或与MMP15及PICK1基因的表达量有关。MMP15 and PICK1 genes are important influencing factors in mammalian gonadal tissues that affect normal physiological functions of gonadal glands.However,their expression pattern in the testicular tissue of Qianbei Ma sheep is still unclear.In order to study the expression of MMP15 and PICK1 genes in the testis of Qianbei Ma sheep at different months of age,this experiment took Qianbei Ma sheep as the research object,detected the expression levels of MMP15 and PICK1 genes in the testis of Qianbei Ma sheep at 1,3,6,9 and 12 months of age by qRT-PCR,and established the standard curve of gene expression.The results showed that MMP15 and PICK1 genes were expressed in testicular tissues of Qianbei Ma sheep at different ages.The expression of MMP15 gene in the testis of Qianbei Ma sheep at different months from high to low was 12 months old>1 month old>9 months old>3 months old>6 months old.There was no significant difference between the age of 12-month-old and 1-month-old(P>0.05),but both of these two months were significantly higher than those of 3 months,6 months,and 9 months(P<0.01).Nine-month-old expression was significantly higher than 3-month-old(P<0.05),and extremely higher than 6-month-old(P<0.01).Three-month-old expression was significantly higher than 6-month-old(P<0.01).The expression levels of PICK1 gene in testis of different ages of Qianbei Ma sheep are ranked from high to low:3 months old>6 months old>12 months old>9 months old>1 month old.The expression levels of 3 and 6 months old was significantly higher than those of 9 and 12 months old(P<0.01).Compared with other months of age,the expression level of 1 month old was significantly lower than those of other months(P<0.01).According to the physiological function development of Qianbei Ma sheep,Qianbei Ma sheep has basically reached sexual maturity at the age of 6 months,so the cell activity in testicular is enhanced and testicular function is active at this time.The results of this experiment suggest that the normal performance of testicular spermgene

关 键 词：黔北麻羊 MMP15 PICK1 相对表达量 睾丸 

分 类 号：S827[农业科学—畜牧学]