Lack of fitness cost and inheritance of resistance to abamectin based on the establishment of a near-isogenic strain of Tetranychus urticae  

作　　者：ZHANG Yan TIAN Tian ZHANG Kun ZHANG You-jun WU Qing-jun XIE Wen GUO Zhao-jiang WANG Shao-li 

机构地区：[1]State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China [2]College of Agriculture,Yangtze University,Jingzhou 434025,P.R.China [3]Sanya Nanfan Research Institute,Hainan University,Sanya 572024,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第6期1809-1819,共11页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China (32072458);the earmarked fund for China Agriculture Research System (CARS-25);the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables, China, and the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIPIVFCAAS)。

摘　　要：Many populations of the two-spotted spider mite, Tetranychus urticae Koch, have developed high levels of resistance to the pesticide abamectin in China and other countries. This study developed a near-isogenic line to understand better the inheritance, cross-resistance, and fitness costs associated with abamectin resistance in the field population of T. urticae in China. We introduced the trait that confers extremely high abamectin resistance in a field-collected population of T. urticae into a susceptible laboratory strain(IPP-SS) to generate an abamectin-resistant near-isogenic line(NIL-Aba).This process was carried out through multiple backcrossing to IPP-SS and via parthenogenesis and abamectin screening. Compared with IPP-SS, the NIL-Aba strain had a 25 147-fold resistance to abamectin and a high level of cross-resistance to bifenthrin(288.17-fold), an intermediate level to emamectin benzoate(42.57-fold), and low levels to bifenazate, chlorfenapyr, cyflumetofen, cyenopyrafen, and cyetpyrafen with resistance ranging from 3.18-to 9.31-fold.But it had no cross-resistance to profenofos. The resistance to abamectin in NIL-Aba was autosomal, incompletely dominant, and polygenic. Based on two sex life table parameters, no fitness cost was found in NIL-Aba. Establishing the NIL-Aba strain provides a reliable basis for an in-depth study of abamectin resistance in T. urticae. New information on toxicological characteristics and fitness cost should facilitate the management of abamectin resistance in field populations of T. urticae.

关 键 词：two-spotted spider mite abamectin resistance near-isogenic strain inheritance pattern fitness cost 

分 类 号：S433[农业科学—农业昆虫与害虫防治]