降香提取物对脂多糖诱导的RAW264.7细胞的抗炎、抗氧化作用和对人毛乳头细胞的促毛发生长作用  

Anti-inflammation and anti-oxidant effect of Dalbergia Odorifera T.Chen extract on lipopolysaccharide-induced RAW264.7 cells and its hair growth-promoting effect on human dermal papilla cells

作　　者：林婷 陈媛媛 马庆 赵斌斌 吴华裕 李文宇[1] 范润哥 温斯健[1] 林有坤[1] Lin Ting;Chen Yuanyuan;Ma Qing;Zhao Binbin;Wu Huayu;Li Wenyu;Fan Runge;Wen Sijian;Lin Youkun(Department of Dermatology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;Guangxi Helanmo Biotechnology Co.,Ltd.,Nanning 530219,China)

机构地区：[1]广西医科大学第一附属医院皮肤性病科,南宁530021 [2]广西鹤兰墨生物科技有限公司,南宁530219

出　　处：《广西医科大学学报》2023年第5期871-876,共6页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.2020JJA140075)。

摘　　要：目的:探讨降香提取物(DOE)对脂多糖(LPS)诱导的RAW264.7细胞的抗炎及抗氧化作用及其对人毛乳头细胞(h DPCs)的促毛发生长作用。方法:将RAW264.7细胞分为空白对照组、模型组、4%DOE组和8%DOE组。空白对照组不予处理,模型组使用LPS诱导体外炎症模型,4%DOE组和8%DOE组用LPS处理后分别加入4%和8%的DOE,分别采用Griess法和酶联免疫吸附试验(ELISA)法测定各组细胞培养上清液中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)的含量,DCFH-DA荧光探针检测各组细胞内的活性氧(ROS)含量。体外培养人毛囊分离的hDPCs,设置空白对照组、4%DOE组和8%DOE组,采用CCK-8法检测细胞活力,RT-qPCR法检测毛发生长因子CTNNB1和毛发抑制因子DKK1基因的表达,western blotting法检测β-连环蛋白(β-catenin)蛋白表达水平。结果:与模型组相比,4%DOE组和8%DOE组RAW264.7细胞NO、TNF-α、ROS含量降低(均P<0.05)。与空白对照组相比,4%DOE组和8%DOE组hDPCs细胞活力升高,CTNNB1基因和β-catenin蛋白表达上调,且DKK1基因表达下调(均P<0.05)。结论:DOE对LPS诱导的RAW264.7巨噬细胞具有抗炎和抗氧化作用,并且可以通过增加hDPCs的活力及调节关键基因的表达促进毛发生长。Objective:To explore the anti-inflammatory and anti-oxidant effect of the Dalbergia odorifera T.Chen extract(DOE)on lipopolysaccharide(LPS)-induced RAW264.7 cells and the hair growth-promoting effect on human dermal papilla cells(hDPCs).Methods:RAW264.7 cells were divided into blank control group,model group,4%DOE group and 8%DOE group.The blank control group was untreated,the model group was treated with LPS to induce the inflammatory model in vitro,and the 4%DOE and 8%DOE groups were treated with LPS and then treated with 4%DOE and 8%DOE,respectively.The contents of nitric oxide(NO)and tumor necrosis factor-α(TNF-α)in the supernatants of cell culture in each group were determined by Griess assay and enzyme-linked immunosorbent assay(ELISA),respectively.The contents of intracellular reactive oxygen species(ROS)in each group were detected by DCFH-DA fluorescent probe.HDPCs isolated from human hair follicles were cultured in vitro,and were divided into the blank control group,the 4%DOE and 8%DOE groups.The viability of hDPCs was detected by CCK-8 assay.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was performed to detect the expressions of hair-growth factor CTNNB1 and hair inhibition factor DKK1.Western blotting was performed to detect the expression ofβ-catenin protein.Results:Compared with the model group,the NO,TNF-α,and ROS contents in RAW264.7 cells in the 4%and 8%DOE groups decreased(all P<0.05).Compared with the blank control group,hDPCs cell viability increased,CTNNB1 gene andβ-catenin protein expression were up-regulated,and DKK1 gene expression was down-regulated in the 4%and 8%DOE groups(all P<0.05).Conclusion:DOE has anti-inflammatory and anti-oxidant effect on LPSinduced RAW264.7 macrophages,and it can promote the hair growth by increasing the viability of hDPCs and regulating the expression of key genes.

关 键 词：降香 抗炎 活性氧 毛发生长 雄激素性秃发 

分 类 号：R285.5[医药卫生—中药学]