氧化石墨烯与氮掺杂氧化石墨烯量子点负载去氧地胆草内酯抑制肿瘤细胞的研究  

Study on the Inhibition of Tumor Cells by Deoxyelephantopin Loaded on Graphene Oxide Versus Nitrogen-doped Graphene Oxide Quantum Dots

作　　者：韩欣彤 曹阳[1,2] 文峰 高助威[3] 李成欣 于晓龙 HAN Xintong;CAO Yang;WEN Feng;GAO Zhuwei;LI Chengxin;YU Xiaolong(School of Materials Science and Engineering,Hainan University,Haikou 570228,China;Key Laboratory of Child Cognition&Behavior Development of Hainan Province,Qiongtai Normal University,Haikou 571127,China;School of Chemical Engineering and Technology,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学材料科学与工程学院,海口570228 [2]琼台师范学院海南省儿童认知与行为发展重点实验室(筹),海口571127 [3]海南大学化学工程与技术学院,海口570228

出　　处：《材料导报》2023年第14期1-7,共7页Materials Reports

基　　金：海南省省级平台项目海南省生物医学材料研发创新服务平台(筹)资助项目(RZ2000007005)。

摘　　要：以膀胱肿瘤细胞UMUC-3为模型,研究了氧化石墨烯(GO)和氮掺杂氧化石墨烯量子点(NGQD)负载抗癌药物去氧地胆草内酯(DEO)体外抑制肿瘤细胞的效果。分别用Hummers法制备GO,球磨法制备NGQD,并通过超声负载的方法将DEO与GO、NGQD进行接枝。采用傅里叶变换红外光谱仪分析DEO与GO、NGQD的接枝机理,通过扫描电子显微镜分析其微观结构和元素分布。使用MTT比色法测试肿瘤细胞在GO-DEO、NGQD-DEO作用下的增殖情况,使用免疫荧光技术进一步分析NGQD-DEO抑制率更高的原因。结果表明,GO与NGQD均可通过共价键接枝的方式负载DEO,同时对比得到,GO-DEO、NGQD-DEO负载组均可通过破坏细胞骨架的方式提高对肿瘤细胞的抑制率,其中NGQD负载15μmol/L DEO且时间为48 h和72 h时对肿瘤细胞骨架的破坏效果最佳。Using bladder tumor cell UMUC-3 as a model,the anti-tumor activity of deoxyelephantopin(DEO)loaded on graphene oxide(GO)and nitrogen-doped graphene oxide quantum dots(NGQD)in vitro was studied.GO was prepared by Hummers method,NGQD was prepared by ball milling method,and DEO was connected onto GO and NGQD by ultrasonic loading.The grafting mechanisms of GO-DEO and NGQD-DEO were analyzed by FTIR.The microstructure and element distribution of GO-DEO and NGQD-DEO were analyzed by scanning electron microscope.MTT colorimetry was used to test the cell proliferation induced by GO-DEO and NGQD-DEO,and immunofluorescence was used to further analyze the reasons for NGQD-DEO inhibition.The results show that both GO and NGQD can load DEO by covalent bond grafting.In comparison,GO-DEO and NGQD-DEO groups can enhance the inhibition of tumor cells by destroying the cytoskeleton,with NGQD loading 15μmol/L DEO for 48 h and 72 h were the best time to destroy the cytoskeleton.

关 键 词：药物载体 去氧地胆草内酯 膀胱癌细胞 细胞增殖 细胞骨架 

分 类 号：R318.08[医药卫生—生物医学工程]