不同外源性物质对园参生长影响的研究  

Effects of different exogenous regulators on cultivated Ginseng Radix et Rhizoma

作　　者：彭娟[1] 张海鸣[1] 丁珊珊 闫寒[1] 范斌[1] 聂颖兰[1] PENG Juan;ZHANG Haiming;DING Shanshan;YAN Han;FAN Bin;NIE Yinglan(Experimental Research Center,China Academy of Chinese Medical Sciences,State Key Laboratory Breeding Base of Dao-Di Herbs,Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases,Beijing 100700,China;Yidi International Agriculture(Jiangxi)Co.,Ltd,Ruijin,Jiangxi 342500,China)

机构地区：[1]中国中医科学院医学实验中心,道地药材国家重点实验室培育基地,中医药防治重大疾病基础研究北京市重点实验室,北京100700 [2]江西益地生物科技有限公司,江西瑞金342500

出　　处：《上海中医药杂志》2023年第6期88-95,共8页Shanghai Journal of Traditional Chinese Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(zz2017005,JJPY2022007)。

摘　　要：目的筛选在人工种植条件下,可诱导人参植株抗性、刺激其产生次级代谢产物的外源性物质。方法基于可诱导的植物防卫反应,对2年生、3年生、4年生人参植株施以水杨酸、壳聚糖、氨基酸等外源性物质,采集人参植株,采用超高效液相色谱-二极管阵列检测器(UPLC-PDA)法测定其主根中次级代谢产物人参皂苷Rg_(1)、Re、Rb_(1)、Rb_(2)、Rb_(3)、Rd的含量,观察处理前后单株鲜重和人参皂苷含量的变化情况。结果验证了生长/分化平衡假说(GDBH),提示增产和优质是一个矛盾;外源性物质氨基酸、水杨酸、壳聚糖可诱导植物防卫反应,启动次级代谢,促进人参皂苷积累。结论水杨酸、壳聚糖和氨基酸3种外源性物质的复合营养是提高园参中人参皂苷含量最优的外源性调节物质。Objective To screen the exogenous regulators that can induce resistance and stimulate Ginseng Radix et Rhizoma plants to produce secondary metabolites under artificial planting conditions.Methods Based on the inducible plant defense response,2-year-old,3-year-old and 4-year-old Ginseng Radix et Rhizoma plants were treated with exogenous substances such as salicylic acid,chitosan and amino acids.Ginseng Radix et Rhizoma plants were collected and the contents of ginsenosides Rg_(1),Re,Rb_(1),Rb_(2),Rb_(3) and Rd in their main roots were determined by ultra high performance liquid chromatography with diode array detector(UPLC-PDA).The changes of fresh weight and ginsenoside content of single plant before and after treatment were observed.Results The growth/differentiation balance hypothesis(GDBH)was verified,suggesting that there is a contradiction between yield increase and high quality,and exogenous substances such as amino acids,salicylic acid and chitosan can induce plant defense response,start secondary metabolism and promote ginsenoside accumulation.Conclusion The compound nutrition of amino acids,salicylic acid and chitosan is the optimal exogenous regulator to effectively improve the fruit quality of cultivated Ginseng Radix et Rhizoma.

关 键 词：园参 人参皂苷 次级代谢产物 活性成分 中药研究 

分 类 号：S567.51[农业科学—中草药栽培]