枇杷果实采前皱缩的转录组分析  

Transcriptome analysis of pre-harvest fruit shrinkage in loquat

作　　者：常晓晓[1] 彭程 陈慧琼 陆育生 邱继水[1] CHANG Xiaoxiao;PENG Cheng;CHEN Huiqiong;LU Yusheng;QIU Jishui(Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture and Rural Affair/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,Guangdong,China)

机构地区：[1]广东省农业科学院果树研究所·农业农村部南亚热带果树生物学与遗传资源利用重点实验室·广东省热带亚热带果树研究重点实验室,广州510640

出　　处：《果树学报》2023年第7期1342-1353,共12页Journal of Fruit Science

基　　金：广东省现代农业产业技术体系创新团队建设项目(优稀水果产业)(2022KJ116)。

摘　　要：【目的】了解早钟6号枇杷果实采前遇到高温天气出现皱缩现象的分子机制,为培育抗皱缩枇杷品种奠定理论基础。【方法】以易皱缩的早钟6号枇杷果实和抗皱缩的思贺大果枇杷果实为材料,对早钟6号枇杷采前不同皱缩程度的果实(正常果ZZS1、轻度皱缩果ZZS2和皱缩果ZZS3)进行生理生化指标分析,并对思贺大果枇杷和早钟6号枇杷果实进行转录组测序,分析早钟6号枇杷不同程度皱缩果实之间以及与思贺大果枇杷之间的差异基因表达情况,筛选与枇杷果实皱缩相关的基因。【结果】早钟6号枇杷皱缩果(ZZS2、ZZS3)与正常果(ZZS1)相比,丙二醛和脯氨酸含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均显著升高。对转录组测序结果分析发现,可能与枇杷果实采前皱缩相关的基因主要参与植物激素信号转导途径、苯丙烷生物合成途径、淀粉和蔗糖代谢途径、油菜素内脂生物合成和信号转导等代谢途径。参与苯丙烷生物合成的12个基因中,有9个与木质素合成相关,在DG vs ZZS1比较中均上调表达;参与植物激素信号转导途径的13个基因中,有6个(2个GH3和4个SAUR)参与生长素信号转导;2个基因(EVM0023097和EVM0032997)参与油菜素内脂生物合成途径,在DG vs ZZS1、ZZS1 vs ZZS2和ZZS1 vs ZZS3三组比较中表达量均上调;NAC转录因子基因在ZZS1 vs ZZS2、ZZS1 vs ZZS3、ZZS2 vs ZZS3三组比较中表达量均上调。结果表明,生长素和油菜素内脂这两种植物激素可能参与枇杷果实采前皱缩过程,木质素合成相关基因可能与不同枇杷品种应对高温抗皱缩能力有关,NAC转录因子可能参与调控枇杷果实皱缩相关基因的表达。【结论】木质素可能与枇杷不同品种应对高温胁迫的能力相关,生长素和油菜素内脂可能在枇杷果实应对高温胁迫的过程中起作用。【Objective】The objective of the experiment is to understand the molecular mechanism of fruit shrinkage of Zaozhong 6 loquat when it was encountering high temperature before harvest,so as to lay a theoretical foundation for cultivating anti-shrinkage loquat varieties.Pre-harvest fruit shrinkage is affected by environmental factors,varieties and hormone levels in the tree.There are few studies on the molecular mechanism of pre-harvest fruit shrinkage and the screening and cultivation of anti-shrinkage varieties.A pre-harvest non-wrinkling loquat variety Sihedaguo was screened out from the resource nursery by our research group.It was used as an anti-wrinkling variety that was compared with Zaozhong 6 to preliminarily analyze the molecular mechanism of the difference in anti-wrinkling ability among the varieties.【Methods】Zaozhong 6(easy to wrinkle)and Sihedaguo(anti-winkling)loquat fruits were selected as the materials.The physiological[malonaldehyde(MDA)and proline]and biochemical[superoxide dismutase(SOD)and peroxidase(POD)]indexes of pre-havest Zaozhong 6 loquat fruits with different shrinkage degrees(normal fruit labeled as ZZS1,slightly shrunken fruit as ZZS2 and shrunken fruit as ZZS3)were analyzed.Transcriptome sequencing of Sihedaguo(DG)and Zaozhong 6 loquat fruits with different shrinkage degrees was carried out.The differentially expressed genes among different degrees of shrunken fruits of Zaozhong 6,and differentially expressed genes between Sihedaguo and Zaozhong 6 normal fruits were analyzed,so as to screen the genes related to fruit shrinkage in loquat.【Results】Compared with the normal fruit(ZZS1),the contents of MDA and proline,and the activity of SOD and POD in the shrunken fruits(ZZS2,ZZS3)of Zaozhong 6 significantly increased.The content of proline and the activity of POD in ZZS3 increased by 81.9%and 94.5%,respectively compared with ZZS1.Four groups of samples(DG,ZZS1,ZZS2 and ZZS3)were subjected to transcriptome sequencing analysis.A total of 76.26 Gb Clean Data was obtained,and the

关 键 词：枇杷 果实 皱缩 高温 转录组 

分 类 号：S667.3[农业科学—果树学]